Genome-wide analysis of the auxin/indoleacetic acid (Aux/IAA) gene family in allotetraploid rapeseed (Brassica napus L.)
首发时间:2017-05-11
Abstract:Auxin/Indoleacetic acid (Aux/IAA) genes participate in the auxin signaling pathway and play key roles in plant growth and development. Although the Aux/IAA gene family has been identified in many plants, within allotetraploid Brassica napus little is known. In this study, a total of 119 non-redundant Aux/IAA genes were found in the genome of B. napus. They were distributed non-randomly across all 19 chromosomes and other non-anchored random scaffolds, with a symmetric distribution in the A and C subgenomes. Evolutionary and comparative analysis revealed that 111 (94.1%) B. napus Aux/IAA genes were multiplied due to ancestral Brassica genome triplication and recent allotetraploidy from B. rapa and B. oleracea. Phylogenetic analysis indicated seven subgroups containing 29 orthologous gene sets and two Brassica-specific gene sets. Structures of genes and proteins varied across different genes but were conserved among homologous genes. Furthermore, analysis of transcriptional profiles revealed that the expression patterns of Aux/IAA genes in B. napus were tissue dependent. Auxin-responsive elements tend to be distributed in the proximal region of promoters, and are significantly associated with early exogenous auxin up-regulation. This comprehensive analysis provides a deeper understanding of diversification of Aux/IAA gene family and will facilitate further dissection of Aux/IAA gene function in B. napus.
keywords: Brassica napus Aux/IAA gene Evolutionary analysis Expression pattern Auxin response
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甘蓝型油菜Aux/IAA基因家族的全基因组分析
摘要:本研究利用生物信息学的方法在甘蓝型油菜基因组中鉴定到119个Aux/IAA基因。这些基因非随机地分布于全基因组的19个染色体和其他未定位的scaffolds,并且在A 和C亚基因组之间匀称分布。进化与比较分析发现,甘蓝型油菜在历史上经历的芸薹属基因组三倍化事件以及异缘四倍化事件导致了111(94.1%)Aux/IAA基因以多拷贝的形式存在。系统进化分析将这些基因分成了7个组,包含29个同源基因群和2个芸薹属特异的基因群。基因结构和蛋白质结构域分布在不同的Aux/IAA基因间存在很大差异。转录组测序分析表明Aux/IAA基因在不同的组织间表达模式存在很大差异。生长素响应元件倾向分布于启动子区域的近端,且与Aux/IAA基因对外源生长素的响应有很大关系。
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No.4732547119994714****
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