The changes in soil microbial communities during and after reductive soil disinfestation

• 1、School of Geography Science, Nanjing Normal University, Nanjing 210023

Abstract：The changes of soil microflora before and after reductive soil disinfestation (RSD) were studied by Miseq high-throughput sequencing and real-time PCR. Results showed that RSD not only significantly increased the microbial populations, but also considerably shaped the soil microflora. Particularly, the relative abundance of phylum Firmicutes significantly increased in the process of RSD and became the predoimant bacterial phylum, which mainly comprised the genera Ruminococcaceae, Coprococcus, and samall group of Clostridium. When the soil returned to the aerobic state, the relative abundance of Firmicutes gradually decreased, replaced by the Acidobacteria, Bacteroides, and Planctomycetes. For fungi, the population of Fusarium oxysporum was considerably decreased during RSD process, leading to a remarkable reduction in relative abundance of phylum Ascomycetes. However, some other fungi of the phylum Ascomycetes, such as the genera Chaetomium and Penicillium, as well as the family Clamidae belonging to the phylum Basidiomycota, were significantly increased in relative abundance. After 60 days aerobic cultivation after RSD treatment, the genus Mycelia became the domiant fungal speices and the relative abundance of Fusarium oxysporum was still reduced. In summary, the restructured soil microflora after RSD treatment make the soil more resistant to soil-borne disease and also benefical to nutrient cycling and plant growth than the untreated soil.

Keywords： Soil Soil-borne disease Microbial community