The expression of hydrogen sulfide synthase in neural stem cells of SVZ and the effect of hydrogen sulfide on proliferation of neural stem cells

• 1、Institute of Neuroscience,Soochow University,Suzhou,Jiangsu Province,215123,China

Abstract：To study the possible effects of H2S on neural stem cells proliferation in mouse subventricular zone (SVZ). Neural stem cells (NSCs) were isolated from SVZ of E13-14 C57BL mouse. For neurosphere counting experiment, the embryonic NSCs of the 2nd to 4th passage were used in this study. The NSCs specific markers nestin and Sox2 were used for identification and labeling of NSCs in immunofluorescent study. And the result showed that about 99% of the isolated embryonic NSCs were positively stained with NSCs specific markers nestin and Sox2. Then immunofluorescent staining, western blot and RT-PCR were applied to identify the distribution and expression of H2S-producing enzymes including cystathionine beta synthase (CBS) and cystathionine gamma-lyase (CSE) in embryonic NSCs, adult SVZ area of C57BL mice and in the brain of embryonic E14, which revealed CBS expression and its co-localization with nestin in the adult SVZ of C57BL mice, as well as in vitro cultures of NSCs. CSE was detected in the brain of embryonic E14 and in vitro cultures of embryonic NSCs. At last, Brdu incorporation assay in combination with immunofluorescent study were applied to detect the proliferation of NSCs in adult mice following treatment with H2S donors NaHS, while the data showed that H2S donors promoted the proliferation of NSCs in mouse SVZ.

Keywords： neurobiology hydrogen sulfide neural stem cell proliferation, cystathionine beta synthase cystathionine gamma- lyase