泥鳅皮胶原蛋白的提取及结构表征
首发时间:2017-06-15
摘要:【目的】用酸法和酶法提取泥鳅皮中胶原蛋白并对其进行表征。【方法】以泥鳅皮为原料提取皮中的酸溶性胶原蛋白和酶溶性胶原蛋白,二者的提取率分别为22.42%和27.32%。利用SDS-PAGE凝胶电泳,肽指纹质谱,傅里叶变换红外光谱,紫外扫描和圆二色谱对其进行结构表征,【结果】实验结果表明提取的胶原蛋白为典型I型胶原蛋白且具有完整的三重螺旋结构。此外测定其氨基酸组成及理化性质,结果表明酸溶性胶原和酶溶性胶原的亚基氨基酸组成为212/1000残基,193/1000残基,变性的温度为36.03℃和33.61℃,等电点为6.42和6.51。
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Extraction and characterization of acid-soluble and pepsin-soluble collagen from skin of loach
Abstract:[Objective] Extraction and characterization of acid-soluble and pepsin-soluble collagen from skin of loach. [Methods] Acid-soluble collagen (ASC) and pepsin-soluble (PSC) were extracted from loach skin. The yields of ASC and PSC were 22.42% and 27.32%, respectively. Sodium dodecyl sulfate-polyacrylamide-gel electrophoresis and peptide mass fingerprint Fourier transform infrared spectrometry, UV measurements and circular dichroism analysis confirmed that[Results] revealed that loach skin contained type I collagen and had a triple helical structure. Moreover there were 212 imino acids/1,000 residues in ASC and 193 imino acids/1,000 residues in PSC. The denaturation temperatures of ASC and PSC were 36.03℃ and 33.61℃, respectively. Zeta potential analysis revealed that net zero charge values of ASC and PSC were 6.42 and 6.51, respectively. Therefore, loach skin collagen may be an alternative to terrestrial mammalian collagen and may enhance the added value of this fish species.
Keywords: collagen characterization peptide mass fingerprint circular dichroism loach skin
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