斑马鱼脾不统血证模型建立与健脾益气摄血方止血疗效研究

高宠; 王佳; 朱长乐; 王冲; 陈信义

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斑马鱼脾不统血证模型建立与健脾益气摄血方止血疗效研究

首发时间：2017-08-10

高宠 ¹
高宠,男,在职博士,中西医结合防治血液病
王佳 ¹ 朱长乐 ¹ 王冲 ¹ 陈信义 ¹
陈信义（1954-）男，博士生导师，中西医结合防治血液病

1、北京中医药大学东直门医院,北京 100700

摘要：目的：建立斑马鱼脑部与心脏出血两种动物模型，分析是否与临床的脾不统血证具有一致性，并观察健脾益气摄血止血效果，为临床推广应用提供依据。方法：第一批实验将野生型 AB 系 60尾斑马鱼随机分正常与模型两组，正常组常规饲养，模型组以50μM辛伐他汀处理24小时。第二批实验：将黑色素等位基因突变型Albino品系斑马鱼分正常组、模型组（常规饲养）及A、B药物实验组，以血流量、运动速度、出血发生率与出血改善率为观察指标，并分析相关数据。结果：①出血发生率：第一批脑部出血发生率75%。第二批心脏出血发生率为65%。②血流量测定：两批实验与正常组比较，模型组血流明显下降（p<0.001）。③运动速度：第一批实验与正常组比较，模型组斑马鱼运动速度明显降低（p<0.001）。④出血改善率：单纯健脾益气方对斑马鱼心脏出血无防治效果；健脾益气摄血方能降低斑马鱼心脏出血发生率，提高出血改善率。结论：①辛伐他汀诱导的斑马鱼出血模型与脾不统血证临床表现基本一致。②健脾摄血方能降低辛伐他汀诱导斑马鱼心脏出血发生率，提高出血改善率。

关键词：脾不统血证斑马鱼出血模型健脾益气摄血出血改善率

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Establishment of zebrafish syndrome model caused by failure of the spleen to control blood, and research on hemostasis effect of spleen-invigorating, qi-replenishing and blood-arresting formula

GAO Chong ¹
高宠,男,在职博士,中西医结合防治血液病
WANG Jia ¹ ZHU Changle ¹ WANG Chong ¹ CHEN Xinyi ¹
陈信义（1954-）男，博士生导师，中西医结合防治血液病

1、Dongzhimen Hospital Affiliated to Beijing University of Chinese Medicine, Beijing 100700

Abstract：OBJECTIVE：To establish zebrafish brain hemorrhage and heart hemorrhage models respectively, analyzing whether the models are in common with the clinical syndrome of failure of the spleen to control blood; to investigate the hemostasis effects of spleen-invigorating, qi-replenishing and blood-arresting formula, supporting its clinical application. METHODS：In the first experiment, 60 AB strain wild type zebrafishes were randomly divided into normal and model group. The model group was treated with 50 μm simvastatin for 24h. The second experiment: The melanin allele mutated Albino strain zebra fishes were divided into normal, model, A group and B group. The observational parameters were as follows: blood flow, velocity of movement, hemorrhage ratio and Improvement ratio of hemorrhage. RESULTS: Hemorrhage ratio: In the first experiment, brain hemorrhage ratio was 75%. In the second experiment, heart hemorrhage ratio was 65%. Blood flow: Compared with the normal group, there was a significantly decrease in the model group (p＜0.001).Velocity of movement: In the first experimental, compared with the normal group, there was a significantly decrease in the model group (p＜0.001).Improvement ratio of hemorrhage: Agents A had little effect in heart hemorrhage of the zebrafish; agents B could reduce heart hemorrhage ratio of the zebrafish, and increase the Improvement ratio of hemorrhage. CONCLUSION: The manifestations of simvastatin-induced zebrafish hemorrhage model are basically similar to that of the clinical syndrome caused by failure of the spleen to control blood. The Spleen-invigorating, Qi-replenishing and Blood-arresting Formula can reduce the heart hemorrhage ratio of zebrafish induced by simvastatin, and increase the Improvement ratio of hemorrhage.

Keywords： Syndrome caused by failure of the spleen to control blood Zebrafish hemorrhage model The Spleen-invigorating, Qi-replenishing and Blood-arresting Formula Improvement ratio of hemorrhage

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高宠，王佳，朱长乐，等. 斑马鱼脾不统血证模型建立与健脾益气摄血方止血疗效研究[EB/OL]. 北京：中国科技论文在线 [2017-08-10]. https://www.paper.edu.cn/releasepaper/content/201708-45.

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论文编号	201708-45
论文题目	斑马鱼脾不统血证模型建立与健脾益气摄血方止血疗效研究
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