Correlation between SmCPS1 promoter polymorphism and tanshinones contents in Salvia miltiorrhiza
首发时间:2017-09-12
Abstract:To investigate the relationship between SmCPS1 promoter polymorphism and the contents of tanshinones. We sequenced the SmCPS1 gene promoter region together with 388bp downstream from the translation start site and the content of tanshinones of 12 different germplasm of Salvia miltiorrhiza. To separate the functional polymorphic loci that may be related with the content of tanshinones from those not, the cis-elements of SmCPS1 promoter were predicted and analyzed by the Plant Transcriptional Regulatory Map database. We found 1) there was a different correlation between the polymorphism in the promoter region and the contents of the tanshinones. 2) Polymorphism on nine cis-elements, an 33bp insertion/deletion sequence and three tandem repeat variations in the sequence of SmCPS1 promoter may be related to the contents of tanshinones. These findings showed the relationships between SmCPS1 promoter polymorphism and tanshinones.
keywords: copalyl diphosphate synthase 1 promoter polymorphism Salvia miltiorrhiza
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丹参SmCPS1启动子区多态性与丹参酮类成分相关性研究
摘要:目的:探索SmCPS1基因启动子区多态性与丹参酮类成分含量间的关系。方法:先通过测序获得不同种质丹参SmCPS1启动子区及转录起始位点下游388bp序列,HPLC法测定其原丹参酮、1,2-二氢丹参酮、丹参酮IIA和丹参酮I的含量;后通过Plant Transcriptional Regulatory Map数据库分析SmCPS1启动子区顺式作用元件以分离出可能影响丹参酮类成分含量的多态性位点;再利用Mantel\' test以探索不同种质丹参SmCPS1启动子区多态性位点与各丹参酮类成分间的相关性。结果:发现1)丹参SmCPS1启动子区多态性与各丹参酮类成分呈不同程度的相关性。2)SmCPS1基因启动子区9个顺式元件上的多态性位点,一段33bp插入/缺失片段及3个串联重复序列变异位点可能与丹参酮类成分的含量相关。结论:丹参SmCPS1启动子区多态性与丹参酮类成分存在相关性。
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