抑制性中间神经元前体细胞移植改善APP/PS1小鼠的认知障碍
首发时间:2017-09-19
摘要:为了探究抑制性中间神经元前体细胞在APP/PS1痴呆模型小鼠大脑移植后迁移分化情况和对小鼠的认知功能的影响。将7月龄雄性小鼠分为3组,WT组,APP/PS1组,APP/PS1移植干细胞组,利用免疫荧光鉴定移植后前体细胞的存活迁移情况和分化为GAD67(glutamic acid decarboxylase 67)、 PV(Parvalbumin)、CR(Calretinin)、NPY(Neuropeptide Y)阳性中间神经元的数量和比例,利用新物体实验和Morris水迷宫检测APP/PS1小鼠学习记忆能力是否改善。免疫荧光结果显示移植后前体细胞具有很好的存活能力,能迁移到整个海马DG区和部分迁移到CA3区,分化成各种类型的GAD67阳性中间神经元。新物体识别结果显示抑制性中间神经元前体细胞移植组对新物体探究时间显著性增加,水迷宫实验显示移植细胞组逃生潜伏期时间和路程显著性减少。因此,抑制性中间神经元前体细胞移植能改善APP/PS1小鼠的认知障碍。?????
关键词: 神经生物学 阿尔茨海默病 中间神经元 前体细胞移植 认知功能
For information in English, please click here
Inhibitory interneuron progenitor transplantation improves cognitive impairment in APP / PS1 mice
Abstract:To investigate the migration and differentiation of inhibitory interneuron progenitor and the effect on cognitive deficiency of APP/PS1 mice after transplantation .The mice were divided into 3 groups: WT group, APP / PS1 group and APP / PS1 transplantation group. The survival and migration of precursors were identified by immunofluorescence with GAD67 (glutamic acid decarboxylase) PV (Parvalbumin), CR (Calretinin), NPY (Neuropeptide Y) antibody. The learning and memory ability of APP / PS1 mice was detected by using the new object experiment and Morris water maze. The results of immunofluorescence showed that the precursors had good survival ability after migration and could migrate to the whole hippocampal region and CA3 region, and differentiate into various types of GAD67 interneuron. The results of new object recognition showed that the time of exploration of the new object was significantly increased in the cells transplantation group. The water maze test showed that the latency and distance of the transplanted cell group were significantly reduced. Thus, inhibitory interneuron progenitor cell transplantation can improve cognitive impairment in APP / PS1 mice.?????
Keywords: Neurobiology Alzheimer\'s disease interneuron progenitor cell transplantation cognitive function
基金:
引用
No.****
同行评议
共计0人参与
勘误表
抑制性中间神经元前体细胞移植改善APP/PS1小鼠的认知障碍
评论
全部评论0/1000