基于GC-MS分析枯草芽孢杆菌抑制变异链球菌生长
首发时间:2017-11-23
摘要:【目的】利用非靶向代谢组学技术检测分析B. subtilis zh78抑制S. mutans生长可能的小分子代谢物,以评估其在龋病防治研究及应用中的前景。【方法】取B. subtilis zh78经生长0h,7h,12h,5d时的菌液,利用牛津杯法将冷甲醇萃取后的代谢物对S. mutans进行抑菌实验;采用气相色谱-质谱联用技术(GC-MS)对代谢物进行检测;利用主成分分析( principal component analysis,PCA) 、正交偏最小二乘判别分析( orthogonal partial least squares, OPLS-DA) 和皮尔森相关分析(Pearson correlation analysis)等方法进行数据分析。【结果】 B. subtilis zh78生长5d时的代谢产物对S. mutans具有明显抑菌作用,其代谢物中,木糖醇、L-谷氨酸及苯乙酸等36种物质与其对S. mutans的抑制作用显著相关。【结论】B. subtilis zh78可能产生木糖醇、氨基酸类及有机酸类等可能抑制S. mutans生长的小分子代谢物,具有一定的防龋应用研究前景。
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Analysis on inhibition effect of B.subtilis on S.mutans based on GC-MS
Abstract: [Objective] This study aimed to determine the possible small molecular metabolite of B. subtilis zh78 that may inhibit the growth of Streptococcus mutans based on untargeted metabolomics approach, and evaluate its prospect in caries prevention and treatment. [Methods] We used the cold methanol to extract the metabolites in the bacteria solutions from the B. subtilis zh78 growth at 0 hour, 7 hours, 12 hours and 5 days respectively. Oxford cup method was used to test the antibacterial activity of the extracted metabolites against Streptococcus mutans. Gas chromatography coupled with mass spectrometry (GC-MS) were applied to metabonomics detection. Then we used principal component analysis (PCA), orthogonal partial least squares discriminant analysis (OPLS-DA) and Pearson correlation analysis to process the data. [Results] The metabolites at 5 days of B. subtilis zh78 showed the most obvious inhibitory effect on Streptococcus mutans. 36 kinds of substances, such as xylitol, L- glutamic acid and phenylacetic acid were produced by B. subtilis zh78, which were significantly related to inhibiting the growth of Streptococcus mutans. [Conclusion] The findings suggested that the B. subtilis zh78 could produce some metabolites (e.g. xylitol, amino acids and organic acids), which might exert the antibacterial effects on Streptococcus mutans. The B. subtilis zh78 displayed its potential in oral probiotic application and the related studies.
Keywords: Bacillus subtilis, Metabolomics, Antibacterial activity, Differential Metabolites
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基于GC-MS分析枯草芽孢杆菌抑制变异链球菌生长
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