Performance of urine proteome in PDX model

• 1、Department of Biochemistry and Molecular Biology，Beijing Normal University, Beijing 100875
• 2、Gene Engineering and Biotechnology Beijing Key Laboratory, Beijing 100875
• 3、Beijing Zhikangboyao Cancer Medicine Research Co., Ltd.

Abstract：In this report,the urine proteome fromPDX model was compared atthe peptide levelin orderto provide clues for the originsof urine proteins in tumor-bearing mice. Urine was collected from the PDX modelmice before and after tumor implantation. The differences of the proteome between the control group and the tumor-bearing group were searched by nanoLC-MS / MS and Label- free proteome quantification. A total of 515 mouse source proteins were identified, of which 8 were differential proteins. 78 human source-derived peptides derived from 42 human proteins were identified in the tumor-bearing group, and the functional annotation of the above proteomic data was performed. The experiment suggestedthat the source of urinary protein in the tumor model was not only the host\'s immune response, but also the protein secreted by the tumor cells. Tumor-derived proteins, which have been found to be secreted by tumor cells through analysis of cellular components, may be involved in the growth and invasion of tumor cells. Study of urine proteome mayprovide clues to not only in explore the process of disease development, but also in related treatment of disease.

Keywords： Proteomics Urine PDX model NanoLC-MS / MS Colorectal cancer