Analysis of the result of syphilis serological tests in a qualified AAA class hospitals in a province in China

• 1、Department of Dermatology, First Affiliated Hospital, Anhui Medical University, 230032

Abstract：【Objective】We Analysis the result of four syphilis serological detection method, including Toluidine red unheated serum test (TRUST) and Treponema pallidum particle agglutination assay (TPPA), Fluorescent treponemal antibody-absorption (FTA-ABS) and western blot (WB) technology, to explore the value of each method in order to provide the more accurate basis for the clinical diagnosis of syphilis. 【Methods】148 Serum samples were collected from syphilis patient in STD laboratory in the department of dermatology of the largest qualified AAA class hospitals in a province in China from 2014.1 to 2017.1. The patients were first detected by TPPA and TRUST methods, and then tested the specific treponema pallidum IgG (TP-IgG) and IgM (TP-IgM) by FTA-ABS and WB methods. 【Result】 1) There were 145 cases positive for the FTA-ABS detection of TP-IgG, and 128 positive for WB detection of TP-IgG. The positive rates of the two methods were 97.97% and 91.89% respectively. 2) Comparing the two methods of TP-IgG with TPPA, the difference was statistically significant betweenTPPA vs. WB and FTA-ABS vs. WB. 3) There was no statistically significant difference between FTA-ABS and WB method to detect TP-IgM. TP-IgM positive rate increased with the TRUST titers increased. 4) There are 7 cases of latent patients whose serum specific TP-IgG test by FTA-ABS and WB were both positive, and the detection TP-IgG of cerebrospinal fluid was also positive. The 7 patients were finally diagnosed with neurosyphilis.【Conclusion】1) To detect the serum specific treponema pallidum, the FTA-ABS and WB methods reached more than 90% positive rate, which provide reliable basis for diagnosing syphilis. 2) When the serum TPPA results was positive, FTA-ABS results can be used to confirm the diagnosis. 3)When the TRUST titer increased, IgM rate increased gradually. The activity level of syphilis can be detected by IgM detection.

Keywords： Syphilis FTA-ABS WB TPPA