Urinary protein changes in early phase of smoking induced Chronic Obstructive Pulmonary Disease rat model

• 1、Gene Engineering Drug and Biotechnology Beijing Key Laboratory,Beijing Normal University,Beijing 100875

Abstract：Chronic Obstructive pulmonary Disease (COPD) is a severe respiratory disease. To identify COPD urinary early biomarkers by proteomics technology may help to reduce mortality rate, to improve patients\' quality of life and to reduce the burden on society. Urine samples from COPD rat model induced by smoking were taken at week 2, week 4 and week 8. By LC-MS/MS, 15 differential proteins with human orthologs were identified. After smoking for 2 weeks when there was no significant pathological changes, 8 differential proteins were identified, two proteins had been reported to be markers of COPD, four proteins were associated with COPD; After smoking for 4 weeks, when slight pathological changes were observed, 7 differential proteins were identified, 3 of them was reported to associate with COPD, 1 protein had been reported to be marker of COPD; After smoking for 8 weeks, there were significant pathological changes, 5 differential proteins were identified , 3 were reported to associate with COPD. The results suggested that the differential urinary proteins might provide important clues for the early diagnosis of COPD.

Keywords： COPD early urinary biomarkers proteomics animal model