模拟糖果生产过程中加热对中草药中总酚含量、抗氧化性及功效成分含量的影响
首发时间:2018-01-12
摘要:本研究的目的是探讨在模拟糖果加工过程中加热对中草药糖果中总酚含量、抗氧化性及主要功效成分含量的影响,以期为中草药糖果的工业化生产提供指导依据。以罗汉果,乌梅,桔梗作为原料,提取浓缩的中草药提取物。将各中草药提取物分别加入溶解的糖液中,在100 C、110 C、120 C、130 C和140 C分别加热10min、20min、30min、40min、60min、120min及180min,对经过不同热处理过程的样品,采用福林酚法测定总酚含量(TPC),用ABTS、DPPH自由基消除能力及亚铁还原能力(FRAP)方法评估抗氧化性,并用HPLC测定主要功效成分含量。实验结果表明,在模拟糖果加工过程中,由于美拉德反应产物的生成及部分结合态多酚化合物的释放,加热使中草药中总酚含量、抗氧化性明显升高,而由于功效成分的热分解,主要功效成分含量降低。140 C/180min加热后,与未加热样品相比,含罗汉果、乌梅、桔梗的糖果中TPC分别增长了约3.5、4.4及4.3倍,而ABTS、DPPH自由基消除能力及FRAP则分别增长了4-8倍,3-5倍及5-6倍,主要功效成分,罗汉果苷V(罗汉果),苦杏仁苷(乌梅)及桔梗皂苷D(桔梗)含量损失率分别为约34.76%,48.44%及44.14%。
关键词: 中草药 加热 总酚含量 抗氧化性 功效成分 模拟糖果生产
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Effects of heating on the total phenolic content, antioxidant activities and main functional components of Chinese herbs during simulated candy processing
Abstract:The aim of this study was to determine the effects of heating on the total phenolic content(TPC), antioxidant activities and content of the main functional component in Chinese herbs during simulated candy processing, and to provide guidance for the industrial production of Chinese herbal confectionery. Using Momordica grosvenori, Ebony and Platycodon as materials, each Chinese herb was extracted and then concentrated to get the extract. Mixed extract with sugar solution well and then heated, the temperature was set at 100 C,110 C,120 C ,130 C or 140 C and the heating time was 10,20,30,40,60,120 or 180 min. Then using the Folin-Ciocalteu reagent assay to determine the total phenolic content (TPC), using ABTS, DPPH scavenging activity and Ferric Reducing Antioxidant Power (FRAP) assay to determine the antioxidant activity and using HPLC to determine the content of main functional component. Results showed that heating can increase the TPC and antioxidant activity of Chinese herbs significantly. Meanwhile, content of the main functional component decreased by heating. Upon heating at 140 C/180min,the TPC of Momordica grosvenori, Ebony and Platycodon increased about 3.5 times,4.4 times and 4.3 times, respectively, compared to the unheated sample, while the ABTS,DPPH scavenging activities and FRAP increased about 4-8 times,3-5 times and 5-6 times, respectively, and content of functional component, Mogroside V (Momordica grosvenori),Amygdalin (Ebony) and PlatycodinD (Platycodon)decreased by 34.76%, 48.44% and 44.14%, respectively
Keywords: Chinese herbs Heating Total phenolic content Antioxidant activity Functional component Simulating candy processing
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模拟糖果生产过程中加热对中草药中总酚含量、抗氧化性及功效成分含量的影响
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