Protective efficacy of Clostrimdium Butyricum on acute pancreatitis and associated intestinal inflammation in mice

• 1、School of Food Science and Technology, Jiangnan University, WuXi, P.R. China 214122
• 2、School of Medicine, Jiangnan University, WuXi, P.R. China 214122

Abstract：Objective To investigate the effects of Clostrimdium Butyricum on acute pancreatitis (AP) and associated intestinal inflammation and explore the potential mechanism. Methods Balb/c mice were randomly divided into control group, AP group, and Clostrimdium Butyricum CGMCC0313.1 (CB0313.1) prophylactic group. The mice in intervention group were treated with CB0313.1 once a day for 21 consecutive days. AP was induced with cearuline on 22st day. The levels of serum amylase and lipase and pancreatic myeloperoxidase (MPO) were determined by relevant commercially available kits, respectively. Pancreas and colon were assayed for inflammatory cytokine using a sandwich ELISA kit. The infiltration of neutrophils and dendritic cells (DCs) in pancreas were examined by flow cytometer. Expression of NOD-like receptor family protein 3 (NLRP3) inflammasome and nuclear factor (NF)-κB in pancreas and colon were determined by western blot. Results Compared with AP group, the mice in CB0313.1 prophylactic group presented significantly reduced serum amylase and lipase levels and pancreatic myeloperoxidase activity, as well as decreased edema and infiltration of neutrophils and DCs in pancreas. The protective effect was also confirmed by decreased inflammatory cytokine in pancreas and colon. Furthermore, CB0313.1 suppress NLRP3 and NF-κB signaling pathway in pancreas and colon. Conclution CB0313.1 protect experimental acute pancreatitis and related intestinal inflammation by improving infiltration of neutrophils and DCs and inhibiting inflammation mediated via NLRP3 inflammasome and NF-κB.

Keywords： acute pancreatitis Clostrimdium Butyricum inflammatory cytokine immunocyte infiltration intestinal inflammation NLRP3 inflammasome