Study on the liver toxicity and reproductive toxicity on male rats by di(2-ethylhexyl) phthalate

• 1、School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu,214122

Abstract：【Objective】In this study, the susceptibility of differentspecies of rat to the liver toxicityand male reproductive toxicity caused by DEHP was analyzed to find out an appropriate animal model for studiesonDEHP toxicity.【Methods】SD and Wistar rats were treated with different doses of DEHP for four weeksduring puberty. The changes of body weight and organs index were observed. The levels of testosterone, ALP, ALT, AST in serum and MDA in liver were measured. Histological changes of liver were also observed. 【Results】After DEHP exposure, body weight and organ quality of SD rats changed significantly compared with control group, while body weight and organ in Wistar rats did not. DEHP had no significant effects on spleen weight and kidney weight in both kinds of rats. In SD rats, there were slight decrease of levels of ALP, ALT and AST in serum during lower dose DEHP exposure, whilst elevated significantly during high dose DEHP exposure.Wistar rats showed elevated level of ALP and ALT during low doses DEHP exposure,but not during high dose exposure. Pathological analysis showed that low doses of DEHP did not cause significant damage to the liver of both kinds of rats, while moderate and high doses of DEHP caused mild fatty degeneration in hepatocytes of rats. Besides, the level of serum testosterone decreased significantly in SD rats but not in Wistar rats. 【Conclusion】SD rats showed more obvious susceptibility to liver toxicity and male reproductive toxicity than that of Wistar ratsto DEHP exposure.

Keywords： DEHP Male Rats Liver toxicity Male reproductive toxicity