口腔鳞状细胞癌外泌体mRNA表达图谱分析
首发时间:2018-03-21
摘要:目的 研究口腔鳞状细胞癌细胞(oral squamous cell cancer cells, OSCC))外泌体与正常口腔上皮细胞(Human immortalized oral epithelial cell, HOEC)外泌体mRNA之间的表达差异,通过特异性外泌体mRNA表达图谱分析,为探究口腔鳞状细胞癌的发生发展机制打下一定的研究基础。 方法 利用高通量测序技术检测CAL-27和HOEC外泌体mRNA表达谱,筛选出差异mRNA,对差异mRNA进行GO(Gene Ontoloty)分析和KEGG通路分析。 结果 口腔鳞状细胞癌细胞外泌体中检测到mRNA 44699 条,口腔上皮细胞外泌体中mRNA数量为44761条,其中,差异表达的mRNA共471条(234个上调,237个下调)。生物信息学分析表明上述差异表达的mRNA参与了口腔癌的生物学调节功能和通路。 结论 口腔鳞癌细胞及口腔上皮细胞均能产生丰富的外泌体;口腔鳞状细胞癌细胞外泌体与正常口腔上皮细胞外泌体间mRNA表达谱存在较大差异,差异表达的mRNA在肿瘤的发生、发展及转移过程中可能有重要作用。
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mRNA expression analysis of exosomes derived from oral squamous cell carcinoma
Abstract:Objectives To compare the mRNA expression between the exosomes derived from oral squamous cell cancer cells (OSCC) and human immortalized oral epithelial cell (HOEC), and further to explore the evolutionary mechanism of OSCC by the approach of bioinformatic analysis. Methods High throughput sequencing technology was used to explore mRNA expression profile in the exosomes of TSCC and HOEC. The differently expressed genes were selected and analyzed by Gene Ontoloty (GO) analysis and KEGG pathway analysis. Results Among 44699 genes detected in the exosomes of OSCC and HOEC,471 differently expressed mRNAs were found (234 mRNAs were up-regulated and 237 mRNAs were down-regulated).Bioinformatics analysis showed that differently expressed mRNAs played an important role in the biological regulation and pathway of OSCC. Conclusion Abundant exosomes can be secreted by OSCC and HOEC. The mRNA expression profiles of exosome from OSCC are quite different compared with that from HOEC, which plays an important role in the occurrence, development and metastasis of tumor. Exploring mRNA expression profiles of the exosomes derived from two different cells can provide new ideas and methods for understanding the evolutional mechanism of oral squamous cell cancer.
Keywords: oral cancer Exosome mRNA High throughput sequencing technology
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口腔鳞状细胞癌外泌体mRNA表达图谱分析
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