Patterned Photonic Nitrocellulose Membrane for Bio-detection Based on Coffee-Ring Effect
首发时间:2018-03-26
Abstract:In this work, we report a method for the fabrication of nitrocellulose (NC) membrane with photonic crystal (PC) pattern for bio-detection. The membrane is prepared by imprinting the corresponding PC pattern into it through thermal Nano-imprint method. Aptamer beacon marked with fluorescein is used for detection of target molecules. Only one droplet (2-3μl) containing the aptamer-target complexes is dripped onto the nitrocellulose membrane. After evaporation (15-20min), the droplet is completely dried and the complexes form a coffee ring on the membrane. The ring keep growing with the increase of complexes concentration .As the aptamer beacon is marked with fluorescein and PC pattern has a fluorescence enhancement effect, the fluorescent signal on the coffee ring for detection is considerable improved. Fluorescent detection of adenosine triphosphate (ATP) is used for testing the application of this method. Owing to fluorescence enhancement effect of PC, the fluorescent signal for detection is improved and the limit of detection is 80.2μM for ATP. Therefore, we believe this method is promising for the fabrication of patterned photonic nitrocellulose membrane for bio-detection and point-of-care testing.
keywords: Analytical Chemistry Photonic crystal;Fluorescence enhancement;Coffee-ring;Nano-imprint;POCT
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基于咖啡环效应的图案化光子晶体硝酸纤维素膜用于生物检测
摘要:在这份工作中我们报道了一种拥有光子晶体(PC)图案的硝酸纤维素膜类纸基底用于POCT生物检测。这种基底是通过热压印技术将对应模板中的光晶图案压印入硝酸纤维素膜并且使用聚多巴胺进行表面改性制备得到的。在检测过程中,标记有荧光分子的适配体信标被用来检测目标分子并且随着目标物浓度提升荧光信号同时增强。只需一滴(2-3μL)包含检测物与分子信标的混合物滴于上述制得的基底上,经过短时间(15-20min)蒸发干燥,混合物由于咖啡环原理会在基底上形成圆环状图案,并且混合物浓度越高,咖啡环越明显。由于信标的末端标记有荧光分子并且基底的光子晶体结构对于荧光有着明显的增强效应,圆环上荧光信号的检测灵敏度得到了可观的提升。为了验证其在生物检测中的应用,我们使用了ATP(三磷酸腺苷)作为检测物。由于光子晶体的荧光增强效应,检测灵敏度得到了很大的提升,其检测线为 80.2μM。因此我们相信这种方法在生物检测学及POCT领域有着良好的前景。
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基于咖啡环效应的图案化光子晶体硝酸纤维素膜用于生物检测
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