基于AU-PDMS复合SERS基底的制备及其在副溶血性弧菌检测中的应用
首发时间:2018-03-30
摘要:本文研究了一种基于表面增强拉曼散射(SERS)的食物病原体副溶血弧菌的检测方法。使用了Apt-Au-PDMS膜组成的纳米结构作为SERS基底。将适配体固定在连接了AuNPs的PDMS膜上作为捕获结构。将适配体和拉曼信号分子4-MBA修饰在AuNPs上,作为SERS感应探针,从而通过适配体与靶标的特异性结合构建了基于拉曼光谱检测的固态的三明治结构。副溶血性弧菌的浓度可以通过测量4-MBA的SERS强度来量化。在最佳条件下,副溶血性弧菌浓度在1.2×102?1.2×106 cfuomL-1范围内与信号强度呈线性相关,检出限为12 cfuomL-1。这种基于超灵敏的适体SERS检测结构其将会是一个有良好前景,适用与许多方面的传感应用。
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PDMS gold nanoparticle composite film as structure for aptamers-based detection of Vibrio parahaemolyticus by surface-enhanced Raman spectroscopy
Abstract:This paper investigated a detection method based surface-enhanced Raman scattering (SERS) of the food pathogen Vibrio parahaemolyticus. Nanostructures consisting of Apt-Au-PDMS film were used as SERS substrates. An aptamer was immobilized on the fuctional PDMS film which covered AuNPs to act as a capture structure. AuNPs were modified with the aptamer and Raman reporter molecule 4-MBA to act as a SERS sensing probes that binds to the target the same way as the Apt-Au-PDMS film. A robust sandwich structure based on Raman spectroscopy is established. The concentration of V. parahaemolyticus can be quantified by measurement of the SERS intensity of 4-MBA. Under optimal conditions,the signal is linearly related to the V. parahaemolyticus concentration in the range between 1.2 × 102 to 1.2 × 106 cfuomL-1, with a detection limit of 12 cfuomL-1.This developed ultrasensitive aptamer-based SERS detection structure suggested that it may be a promising strategy for a variety of sensing applications
Keywords: Pathogenic bacteria PDMS Gold nanoparticles Food safety
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基于AU-PDMS复合SERS基底的制备及其在副溶血性弧菌检测中的应用
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