Effect of nano-TiO2 with different characters on bone formation and immunotoxicity in MC3T3-E1

• 1、School of Biological Science and Medical Engineering,Beihang University,Beijing 100191

Abstract：Object: To evaluate the effect of nano-TiO2 with various physical characters on cell growth, differentiation and immunotoxicity. Methods: Using CCK-8 assay detect the cell proliferation, measuring alkaline phosphatase(ALP) content quantitatively, using enzyme-linked immunosorbent assay(ELISA) measured IL-1,IL-6 content. Result: Nano-TiO2 has inhibitary effect on cell proliferation, and it makes difference with different crystalization. The differentiation effect on MC3T3-E1 also has difference, when the concentration of nanoparticles is low, anatase materials inhibited more. When the concentration of nanoparticles increases, the inhitition effect of rutile materials increases. Nanoparticle crystalization has little connection with IL-1, while it makes difference with IL-6, rutile materials stimulate more serection of IL-6. In the same concentration, the concentration ofIL-1 is in a high level in a shorter incubation time. When the incubation time increases, the concentration of IL-6 in cell culture supernate which cocultured with rutile materials decreases obviously. Conclusions: As one of the artificial implants materials, the cytotoxicity of Nano-TiO2 matarials can\'t be ignored, which is related to crystalization, concentration and time.

Keywords： TiO2 nanoparticle cell proliferation differentiation immunotoxicity