Proteomics Screening Proteins Differentially Expressed in Mouse Skin Tissue Injury by Radon

• 1、School of Radiation Medicine and Protection, Medical college of Soochow University

Abstract：Objective: Epidemiological documentations have shown that there is a certain correlation between Radon exposure and the occurrence of skin-related diseases. However, the effects and mechanisms of Radon on skin tissues have not been reported. The purpose of this study was to study the effects of Radon on skin tissues and changes in protein.?Methods: By establishing a model of mice stained with Radon, the skin lesions of mice were observed by HE staining, β-galactosidase staining and Masson staining. The skin tissue proteins of the stained Radon mouse models were collected and passed through isotopes. Labeled relative and absolute quantification (iTRAQ) technology and HPLC/MS techniques were performed on control/stained skin tissue. Bioinformatics analysis was performed on differential proteins through database search. Result: Radon can cause skin tissue changes and damage in mice, including thinning and defect of stratum corneum, senescence of skin tissue, and morphological changes of mitochondria in skin tissue; Through proteomic analysis, 3,496 differential proteins were screened, of which 45 were upregulated by more than 1.8-fold and 83 were downregulated by 0.83-fold. Bioinformatics analysis showed that the above differential proteins mainly affect multiple pathways, including RNA transport, cysteine and methionine metabolism, amino acid biosynthesis and glutathione metabolism.

Keywords： Ionizing radiation Radon Skin Proteomics