新型胶原基原位水凝胶的制备及其性能研究
首发时间:2018-04-03
摘要:近年来,原位水凝胶材料已成为组织工程领域的研究热点。本文通过马来酰化胶原(Col-MA),巯基化透明质酸(HA-SH)和聚乙二醇二丙烯酸酯(PEGDA)在生理条件下的原位交联制备了新型胶原基原位水凝胶。圆二色谱图证明Col-MA仍然具有完整的三螺旋结构。流变学分析结果表明控制各组分含量可以使水凝胶成胶时间由49.75分钟缩短到18秒,储能模量由30.47 Pa增加到6582.24 Pa。水凝胶能快速溶胀至平衡,含水量达到90%以上。扫描电子显微镜观察结果显示,制备的水凝胶具有均匀的多孔结构,且通透性良好。蛋白质吸收实验表明牛血清白蛋白能逐渐被水凝胶吸收。细胞培养实验结果显示水凝胶具有良好的生物相容性。基于这些优点,这种原位水凝胶在组织工程领域有较好的应用前景。
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Preparation and Properties of Novel Collagen-Based In-situ Hydrogels
Abstract:Recently, in-situ hydrogel has become a hot spot in the field of tissue engineering. In this work, novel collagen-based in-situ hydrogels were prepared through crosslinking of maleic acylated collagen (Col-MA), thiolated hyaluronan (HA-SH) and PEGDA. Circular dichroism (CD) spectras revealed the complete triple helix structure of Col-MA. Rheology analysis indicated that the gelation time decreased from 49.75 min to 18 s and the storage modulus increased from 30.47 Pa to 6582.24 Pa by controlling the content of each component. The hydrogels swelled to equilibrium quickly and the water contents were above 90%. SEM images illustrated that the as-prepared hydrogels possessed uniform porous structure with good permeability. Protein absorption experiment suggested that BSA could be absorbed into hydrogels gradually. Furthermore, the hydrogels showed good biocompatibility. Taken together, these in-situ hydrogels show strong potential in tissue engineering.
Keywords: organic polymer materials in-situ hydrogel collagen tissue engineering
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