Toxic effects on HUVEC cells after exposure to HTO

• 1、School of Radiation Medicine and Protection,Medical College of Soochow University

Abstract：Objective: To investigate the changes of morphology, size, proliferation and senescence of HUVEC cells after exposure to tritium water. Method: HUVEC cells were exposed to tritium water for 15 consecutive generations. The morphology of cells exposed to tritium water was observed under ordinary optical microscope, the surface area of the cells was measured by Image J software. CCK-8 kit was used to detect the change of HUVEC cell proliferation activity at different time points, and β -galactosidase staining kit was used to detect HUVEC cell senescence after tritium water exposure. COOLER algorithm was used to calculate the absorbed dose of tritium water. Results: After exposure to tritium water, the morphology of HUVEC cells changed with the time of exposure, the cytoplasmic particles increased and the boundary was blurred. Cell surface area increased.Cell senescence was observed in T3 and T6 cells.The proliferation activity of T6, T9, T12, T15 cells was inhibited. With the increase of exposure time, the absorbed dose of cell induced by HTO increased. It can be seen that tritium water has cytotoxicity to HUVEC cells.

Keywords： HTO；HUVEC cell；proliferation；senescence