金华佛手精油纳米乳制备及其对HT-29细胞体外增殖的抑制作用
首发时间:2018-04-09
摘要:本文研究了以佛手精油和MCT混合为油相,吐温(Tween-80)为表面活性剂,通过高压均质法制备佛手精油纳米乳液(O/W型)的工艺。并探讨了纳米乳对HT-29细胞增殖的抑制作用。以粒径大小和PDI为指标优选高压均质法参数,采用四甲基偶氮唑蓝(MTT)法检测纳米乳对细胞增殖的抑制影响。研究结果表明,油相质量百分比10%,(佛手精油和MCT质量比4:1),乳化剂的质量百分比2%,在均质压力10000 Psi,均质次数3次的工艺条件下,得到的佛手精油纳米乳粒径大小为100-120 nm,PDI在0.3以下。佛手精油纳米乳对HT-29细胞的增殖有明显的抑制效果,且呈剂量依赖型。
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Preparation of Jinhua Fingered citron Essential Oil Nanoemulsion and Its Inhibitory Effect on Proliferation of HT-29 Cells in Vitro
Abstract:This article studied on the process of preparing fingered citron essential oil nanoemulsion (O/W type) by high-pressure homogenization, using fingered citron essential oil and MCT mixed as oil phase and Tween-80 as surfactant. The inhibitory effect of nanoemulsion on the proliferation of HT-29 cells was explored. The parameters of high-pressure homogenization method were used as the index of particle size and PDI, and the inhibitory effect of nanoemulsion on cell proliferation was detected by MTT assay. The results showPreparation of Jinhua Bergamot Essential Oil Nanoemulsion and Its Inhibitory Effect on Proliferation of HT-29 Cells in Vitroed that the mass percentage of oil phase was 10%, (mass ratio of fingered citron essential oil and MCT was 4:1), the mass percentage of emulsifier was 2%, and it was obtained under the conditions of homogeneous pressure of 10000 Psi and 3 times of homogenization. The fingered citron essential oil nanoemulsion has a particle size of 100-120 nm and a PDI less than 0.3. The fingered citron essential oil nanoemulsion significantly inhibited the proliferation of HT-29 cells in a dose-dependent manner.
Keywords: fingered citron essential oil nanoemulsion Cell apoptosis
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金华佛手精油纳米乳制备及其对HT-29细胞体外增殖的抑制作用
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