BSMV-VIGS技术在小麦抗赤霉病基因鉴定中的应用
首发时间:2018-05-18
摘要:病毒引发的基因沉默(VIGS)在研究功能基因方面具有周期短、宿主范围广的优势。本实验探索了BSMV-VIGS体系在小麦赤霉病上的应用。通过研究发现,在挑旗期接种病毒时接种效率最高,并且沉默效率达到78%。然而,当小麦接种病毒后可能会降低对赤霉病的免疫力。为了降低有病毒本身造成的误差,本实验探究了几种毒性不同的禾谷镰刀菌种。最终,确定SF06-1菌种可以应用到BSMV-VIGS体系在赤霉病抗性鉴定中。此外,本实验利用BSMV-VIGS体系,探究了JA信号路径中的TaAOS基因在赤霉病中发挥的调控作用,并且利用转基因株系进一步验证了TaAOS基因在赤霉病中的积极作用。?????
关键词: 小麦;禾谷镰刀菌;BSMV-VIGS; TaAOS; RNAi
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Application of BSMV induced gene silencing (VIGS) for identification of FHB resistant genes
Abstract:Virus induced gene silencing (VIGS) showed several advantages to identify gene functions such as short experimental cycle, more broad hosts et al. In this study, the BSMV-VIGS system was explored to evaluate Fusarium head blight (FHB) resistance in wheat. With variable conditions tested, it showed that the maximal silencing efficiency 78% on spike was obtained when the recombinant BMSV was inoculated on flag leaf at flagging. However, the plant may reduce its own immunity to FHB when inoculated with BSMV. To induce this impact, different Fusarium graminearum strains was tested and a less toxic isolation was selected for FHB resistance evaluation. Using this system, TaAOS involved inApplication of BSMV induced gene silencing (VIGS) for identification of FHB resistant genes Jasmonic acid (JA) signaling pathway were identified to positively regulate FHB resistance, which was underpinned by the results when silencing TaAOS in wheat by stable transgenic plants.
Keywords: Wheat Fusarium head blight (FHB) BSMV-VIGS TaAOS RNAi
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BSMV-VIGS技术在小麦抗赤霉病基因鉴定中的应用
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