The application of cell 3D culture in the study of tissue engineering

• 1、College of biology, Hunan University, Changsha 420082

Abstract：In recent years, the three-dimensional (3D) culture of cells has gradually become one of the hotspots in the cross field of cell biology and tissue engineering. The main point is to make use of the material with high biocompatibility and good biodegradability to prepare the required porous structure, and to construct a space like cell growth dependent in vitro. The substrate environment enables cells to grow in a three-dimensional way and shows characteristics different from two-dimensional planar culture. 3D cell culture provides a good research platform for the in-depth study of cell growth and behavior characteristics, especially the behavior of tumor cells, as well as the key factors for stem cell proliferation and differentiation, and will be expected to become a transition between traditional planar two-dimensional culture and clinical research in the process of basic medicine in vitro. This article reviews the characteristics of three-dimensional cell culture, and its applications in tissue engineering, tumor research and regenerative medicine.

Keywords： Biomedical engineering tissue engineering 3D culture tumor mechanism