Dynamic Changes of Liquiritin and Expression of Chalcone Isomerase Gene in Glycyrrhiza uralensis Fisch

• 1、 College of Agronomy,Agricultural University of Hebei/North China Key Laboratory for Crop Germplasm Resources of Education Ministry,Baoding 071001
• 2、College of Life Science Agricultural University of Hebei,Baoding 071001

Abstract：Liquiritin is one of the important active ingredients and indicators of quality evaluation in Glycyrrhiza uralensis Fisch. At present, most of the cultivated varieties contain non-compliance with the content of liquiritin. To reveal the dynamic changes and clarify the key gene function is the base for quality breeding. Therefore, this paper focused on the rule of liquritin changes and spatiotemporal expression of CHI dynamic changes. The results showed that liquiritin was distributed in all parts of licorice, and its content in the underground was significantly higher than that in the shoot. The content in the main root was highest in the whole year, and it was the second highest in the roots. Between different years, liquiritin showed an increase in the content of liquiritin as the growth period increased. In the licorice tissues, the relative expression level of CHI gene in leaves was the highest, followed by stems, indicating that the expression levels of CHI gene in different tissues at different stages were different. The results provided the base for further reveal the mechanism of liquiritin synthesis.

Keywords： Glycyrrhiza uralensis Fisch. Liquiritin Spatiotemporal expression