Synthesis and Application of novel fluorescent probe for Hydrogen Sulfide

• 1、Chemistry and Chemical Engineering School,Hunan University,Changsha City Post Code 410082

Abstract：In this paper, a novel ratiometric fluorescence probe is designed and synthesized for the detection of hydrogen sulfide in vivo. The probe is designed by naphthalimide imide as the fluorophore template, (azidomethyl)benzeneas as the recognition site and 4-(2-aminoethyl)morpholine as the targeted groups. Under the 405 nm light excitation, the probe itself has a strong fluorescence emission peak at 450 nm, but with the response with hydrogen sulfide, probe has an obvious fluorescence enhancement at 550 nm and the peak of 450 nm decreased significantly, which shows the characteristics of type ratio fluorescent probe. The probe is sensitive to hydrogen sulfide and the detection limit is ~40 nm. In addition, the probe showed the advantages of high selectivity and high stability under physiological conditions. In addition, the probe also has good low cytotoxicity and biocompatibility, which was successfully used for fluorescence imaging of hydrogen sulfide in living cells.

Keywords： Fluorescence probe Fluorescence imaging Hydrogen sulfide