LiCl/DMAc溶解再生甘蔗渣的表征分析与酶解特性
首发时间:2018-05-30
摘要:采用氯化锂/N,N-二甲基乙酰胺对甘蔗渣和稀硫酸预处理的甘蔗渣溶解后在水中再生,以提高其酶解率,并对原生样品、再生样品和溶解残渣进行组分分析、结构表征和酶解实验。结果表明稀硫酸预处理能够促进甘蔗渣在LiCl/DMAc中的溶解。再生样品不仅组成发生改变,而且结构更加疏松,纤维素构型从Ⅰ型变为Ⅱ型。由于LiCl/DMAc对纤维素、木聚糖、阿拉伯聚糖和木质素的溶出作用导致溶解残渣组成改变,结构更加疏松,结晶指数降低。酶解结果显示,再生样品酶解率显著提高,甘蔗渣再生样品中纤维素和木聚糖酶解率分别为82.18%和51.71%,而经稀硫酸预处理后的甘蔗渣再生样品中纤维素酶解率高达89.04%。此外,溶解残渣中纤维素和木聚糖的酶解率也较溶解前显著提高。采用LiCl/DMAc对甘蔗渣溶解再生可作为一种有效的木质纤维素处理手段。
关键词: 生物化工 生物质 预处理 氯化锂/N,N-二甲基乙酰胺 酶解
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Characterization and enzymatic hydrolysis of sugarcane bagasse dissolved and regenerated in LiCl/DMAc
Abstract:n this paper, sugarcane bagasse and dilute sulfuric acid pretreated sugarcane bagasse were dissolved in lithium chloride/N, N-dimethylacetamide ((LiCl/DMAc) and regenerated in water bath to promote the enzymatic hydrolysis of the materials. The undissolved residue, untreated and regenerated materials were investigated by compositional analysis, characterization analysis and enzymatic hydrolysis. The dissolution ratio of sugarcane bagasse was improved by dilute sulfuric acid pretreatment. The chemical composition of regenerated materials was changed by dissolution and regeneration, and the morphologies of regenerated materials became more porous and the crystal structure of cellulose in regenerated materials changed from I to II. The structure of undissolved residue became looser and the crystalline index decreased due to the dissolution of cellulose, heCharacterization and enzymatic hydrolysis of sugarcane bagasse dissolved and regenerated in LiCl/DMAc solvent systemmicellulose and lignin in LiCl/DMAc. The enzymatic hydrolysis was affected by these changes in chemical composition and morphologies, and the cellulose and xylan digestibilities were improved obviously. The cellulose and xylan digestibilities of regenerated sugarcane bagasse were 82.18% and 51.71%, respectively, and the cellulose digestibility of regenerated sugarcane bagasse pretreated by dilute sulfuric acid was 89.04%. In addition, the digestibilities of cellulose and xylan in the undissolved residue were also improved compared with those in the native materials. Dissolution and regeneration of sugarcane bagasse by LiCl/DMAc was an efficient method for lignocellulose treatment to improve the enzymatic hydrolysis.
Keywords: Biochemical engineering Biomass Pretreatment LiCl/DMAc Enzymatic hydrolysis
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