基于CoOOH增强鲁米诺-过氧化氢化学发光体系实现抗坏血酸的超灵敏检测
首发时间:2018-06-05
摘要:抗坏血酸(AA)是人体进行正常生理活动所必需的重要物质,在许多生化反应中发挥着重要作用。因此,对药用制剂、饮料和食品等中抗坏血酸的定量检测变的至关重要。在这个工作中,基于羟基氧化钴(CoOOH)纳米片层增强传统的鲁米诺-过氧化氢发光体系,一种快速高效且超灵敏检测抗坏血酸的增强化学发光体系被建立。实验结果表明,超薄CoOOH纳米片层作为催化剂显著提高了鲁米诺-过氧化氢的发光强度,增强效果可达1400倍左右。通过优化实验条件,化学发光强度的抑制与抗坏血酸的浓度在1-500 pmol/L范围内呈线性关系,检测限为0.39 pmol/L。同时,本方法实现对实际样本维生素片的检测,展现出在食品药品分析方面的应用潜力。
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Ultrasensitive detection of ascorbic acid by using CoOOH nanosheet to enchance chemiluminescence luminol-peroxide system
Abstract:Ascorbic acid (AA) as an essential vitamin in human body participates in various physiological reactions and plays a key role in many biochemical processes. Therefore, it is of vital importance to monitor and quantify AA in commercial tablets, beverages and food. In this work, a rapid and ultrasensitive chemiluminescence (CL) system for the detection of AA was developed, in which ultrathin cobalt oxyhydroxide (CoOOH) nanosheets was applied in the conventional luminol-hydrogen peroxide CL system. The results showed that ultrathin CoOOH nanosheets as a catalyzer remarkably improved the CL intensity of CoOOH-luminol-hydrogen peroxide system, up to about 1400-fold.Under the optimized conditions, the CL inhibition efficiencies increase linearly with the concentrations of AA in the range of 1-500 pmol/L, and the limit of detection was 0.39 pmol/L. Moreover, the proposed CL system was successfully applied in the determination of AA in medicinal tablets with satisfactory results.
Keywords: Ascorbic acid Chemiluminescence CoOOH nanosheets Luminol
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基于CoOOH增强鲁米诺-过氧化氢化学发光体系实现抗坏血酸的超灵敏检测
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