急性脑梗死患者血小板活化指标CD62P的检测与临床意义分析

吴晓琳; 王丽娜; 姚璐

0
0
浏览
下载

摘要
关键词
基金信息
论文图表
动态公开评议
相关论文
评论

急性脑梗死患者血小板活化指标CD62P的检测与临床意义分析

首发时间：2018-06-14

吴晓琳 ¹
吴晓琳(1983-)，女，硕士，主治医师，电话：13572967346，E-mail：36463909@qq.com
王丽娜 ¹ 姚璐 ²
姚璐（1982-），女，博士，硕士生导师，主要从事神经退行性疾病发病机制及诊疗研究

1、陕西省西安市第九医院神经内科，西安 710054
2、西安交通大学医学部基础医学院生理系与病理生理学系，西安，710061

摘要：目的：本研究通过检测急性脑梗死患者血小板活化指标CD62P的表达变化，探讨其与急性脑梗死患者病情轻重程度的相关性。方法：本研究选择57例急性脑梗死患者和30例非脑血管病患者作为研究对象。采用流式细胞术检测全血血小板表面活化依赖的膜糖蛋白CD62P的表达变化，平均荧光强度代表CD62P的表达量。根据神经功能缺损评分将急性脑梗死组分为轻、中、重型三组，比较不同组间CD62P的表达并与30例非脑血管病对照组比较。数据以均值±标准差的形式表示。用SPSS17.0统计软件进行数据管理与分析，采用单因素方差分析（ANOVA）即F检验，t检验和LSD－t检验，以P<0.05为有统计学意义。结果：急性脑梗死组CD62P的阳性率及平均荧光强度均显著高于对照组（P<0.01），说明急性脑梗死时血小板高度活化。急性脑梗死轻型组、中型组及重型组相比较，组间CD62P的阳性率及平均荧光强度均存在显著性差异，且重型组的血小板CD62P的表达增高最显著（P<0.01）。结论：急性脑梗死患者血小板高度活化，并且随病情加重，血小板活化程度增高。监测全血血小板活化水平，将有利于了解急性脑梗死患者的病情轻重程度，亦可作为急性脑梗死治疗后疗效观察的重要参考指标。

关键词：神经内科急性脑梗死 CD62P 血小板活化

For information in English, please click here

The study on the expression of CD62P in patients with acute cerebral infarction

Wu Xiaolin ¹
吴晓琳(1983-)，女，硕士，主治医师，电话：13572967346，E-mail：36463909@qq.com
Wang Lina ¹ Yao Lu ²
姚璐（1982-），女，博士，硕士生导师，主要从事神经退行性疾病发病机制及诊疗研究

1、Department of Neurology,Ninth Hospital of Xi'an,Xi'an 710054,Shannxi
2、Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Health Science Center , Xi'an Jiaotong University, Xi'an 710061, Shannxi

Abstract：Objectives: We observed the surface membrane glycoprotein CD62P expression of patients\' platelet with acute cerebral infarction in order to explore the significance of platelet activation in clinical trials. Methods: We chose 57 patients with acute cerebral infarction and 30 patients without cerebrovascular disease. We test the expression changes of CD62P in whole blood platelets by flow cytometric detection. We divided the patients with acute cerebral infarction into three groups (as light, medium and heavy groups) according to the degree of neurologic deficits. And we compared the levels of CD62P between these three groups. The date was displayed as the mean ±S.D and the statistical analyses were performed by SSPS17.0 Software. The date were statistically analyzed by one-way ANOVA, the unpaired Student t test and the least significant differences t test (LSD－t). A p-value less than 0.05 were considered as significance. Results: The positive percentage and the fluorescencence intensity of CD62P in patients\' platelets with acute cerebral infarction were significantly higher than those in control group (p<0.01), which illustrated the platelet activation was higher in patients with acute cerebral infarction. The CD62Ppositive expression and average fluorescence intensity of CD62P were significantly difference between three groups with acute cerebral infarction, and the CD62P expression of platelets in heavy group was significantly increased (P < 0.01). Conclusions: The levels of CD62P were related to the neurologic deficits, which reflect the platelet activation levels could monitoring severity of illness and progress of patients with cerebral infarction and. And our results alsoshowed the platelet activation could be used as marker for acute cerebral infraction prevention, clinical observation and prognosis important reference index.

Keywords： Neurology；Acute cerebral infarction；CD62P；Platelets activation

基金：

1. 国家自然科学基金资助（31300899）

论文图表：

引用

导出参考文献

.txt

.ris

.doc

吴晓琳，王丽娜，姚璐. 急性脑梗死患者血小板活化指标CD62P的检测与临床意义分析[EB/OL]. 北京：中国科技论文在线 [2018-06-14]. https://www.paper.edu.cn/releasepaper/content/201806-77.

No.****

动态公开评议

共计0人参与

动态评论进行中

全部评论

0/1000

论文编号	201806-77
论文题目	急性脑梗死患者血小板活化指标CD62P的检测与临床意义分析
文献类型
收录期刊	上传封面中文期刊英文期刊期刊名称（中文）期刊名称（英文）年，卷（）上传封面中文专著英文专著书名（中文）书名（英文）出版地出版社出版年上传封面中文译著英文译著书名（中文）书名（英文）出版地出版社出版年上传封面中文论文集英文论文集编者.论文集名称（中文） [c]. 出版地出版社出版年， - 编者.论文集名称（英文） [c]. 出版地出版社出版年，- 上传封面中文文献英文文献期刊名称（中文）期刊名称（英文）日期-- 在线地址http:// 上传封面中文文献英文文献文题（中文）文题（英文）出版地出版社,出版日期-- 上传封面中文文献英文文献文题（中文）文题（英文）出版地出版社,出版日期--
英文作者写法：中外文作者均姓前名后，姓大写，名的第一个字母大写，姓全称写出，名可只写第一个字母，其后不加实心圆点“.”, 作者之间用逗号“，”分隔，最后为实心圆点“.”, 示例1：原姓名写法：Albert Einstein,编入参考文献时写法：Einstein A. 示例2：原姓名写法：李时珍；编入参考文献时写法：LI S Z. 示例3：YELLAND R L,JONES S C,EASTON K S,et al.