Effects of different doses of buckwheat on hypercholesterolemia mice

• 1、School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122

Abstract：[Objective] Investigate the changes in the lipid and cholesterol metabolism related genes of C57BL/6 mice after the maltodextrin and corn starch replaced with different doses of buckwheat flour in high-cholesterol diet. [Methods] The C57BL/6 mice were fed the AIN-93M standard diet, high-cholesterol model (Paigen) diet, high-cholesterol and low-dose buckwheat diet and high-cholesterol and high-dose buckwheat diet，then measured the serum biochemical index in mice (total cholesterol, triglycerides, high-density lipoprotein cholesterol (hdl-c), low density lipoprotein) and liver tissue morphology observation, the relative expression of liver cholesterol metabolism genes (HMG CoA, srebp-2, CYP7A1, LXR-a and LDL-r) to analyze buckwheat cholesterol-lowering effect. [Results] The high doses buckwheat group die effects of buckwheat on hypercholesterolemia mice of Buckwheat diet on Hypercholesterolemic Micet significantly lowered the LDL-C in the liver homogenate compared with model group and low doses group. The relative expression of HMG-CoA and srebp-2 liver gene was down-regulated and up-regulated in the low-dose group of buckwheat. Compared with the low dose group, high doses buckwheat diet downgraded the relative expression of LXR-a and raise LDL-r in addition. [Conclusion] These data suggests that the consumption of buckwheat may help prevent and treat hypercholesterolemia.

Keywords： buckwheat low density lipoprotein cholesterol metabolism