基于酶失活模型的油包水型大豆磷脂水解研究
首发时间:2018-07-25
摘要:由于酶失活及异相体系的存在影响磷脂水解的过程,机理模型建立困难。本文测定了不同初始酶浓度条件下磷脂酶A1对大豆浓缩磷脂的水解情况,对油包水体系中磷脂酶活性变化进行研究。应用准稳态理论建立模型,分别拟合得到酶一级及二级失活模型,所得二级失活模型具有更高的相关性。以初始酶浓度构建的酶解过程的动力学模型,相关系数R2=0.983,产物生成速率k2=12.88/h,酶失活常数kde2=1.935L/g h,半饱和常数为Ke=74.38g/h,能较好拟合实验数据,对有效调控水解速率,获得不同特性的水解磷脂具有实际指导作用。
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Study on water-in-oil soybean phospholipid hydrolysis based on the model of enzymatic deactivation
Abstract:Since the inactivation of enzymes and the presence of heterophasic systems affect the hydrolysis process of phospholipids, the establishment of a mechanism model is difficult. In this paper, the hydrolysis of soybean phospholipids by phospholipase A1 under different initial enzyme concentrationsweremeasuredand the changes of phospholipase activities in the water-in-oil system were studied. The quasi-steady state theory was used to establish themodel, and the first and second order deactivationmodels of the enzyme were fitted respectively. The resulting secondary inactivation model had a higher correlation. The kinetic model of the enzymatic hydrolysis process constructed could fit the experimental data, and offer practical guidance on effectively regulating the hydrolysis rate and obtaining hydrolyzed phospholipids having different characteristics, with correlation coefficient R2=0.983, product generation rate k2=12.88/h, enzyme inactivation constant kde2=1.935L/g h, half-saturation constant Ke=74.38g/h.
Keywords: Phospholipase A1 Soybean concentrate phospholipid Enzymolysis model
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