表面配体对量子点物理化学性质的影响
首发时间:2018-09-04
摘要:量子点( QDs)的表面配体负责与生物环境相互作用,其类型将大大影响细胞摄取。在本文中,调查表面配体对量子点物理化学性质的影响,并检查不同的量子点的生物成像。通过用短链巯基配体(MPA,半胱氨酸)或PEG衍生物配体(DSPE-PEG)固定CdSe量子点表面来制备三种类型的QDs制剂。然后,进行了研究,以评估制剂光稳定性(吸收光谱、发射光谱、傅里叶红外光谱的测量)和生物成像。实验结果表明:CdSe、DSPE-PEG-CdSe量子点有较高的结晶度,粒径为3~5nm。PEG修饰的量子点吸收峰表现出0nm移动,MPA和CYS修饰的量子点吸收峰分别表现出3nm和5nm的红移。发现QDs的PEG链和表面电荷在细胞摄取中起关键作用,用MCF-7乳腺癌细胞作为细胞摄取模型,发现与阳离子QDs制剂相比,阴离子QDs具有较高的摄取。一般而言,表面配体不同的QDs在某一方面有一定的强度的限制。因此,在实验中应仔细考虑和选择QDs配方的类型,从而将其限制所产生的影响降到最低。
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Effect of Surface Ligands on Physical and Chemical Properties of Quantum Dots
Abstract:The surface ligands of quantum dots (QDs) are responsible for interacting with the biological environment and their type will greatly affect cellular uptake.In this paper, we investigate the effect of surface ligands on the physico-chemical properties of quantum dots and examine the biological imaging of different quantum dots.Three types of QDs formulations were prepared by immobilizing the CdSe quantum dot surface with a short-chain sulfhydryl ligand (MPA, cysteine) or a PEG derivative ligand (DSPE-PEG).Then, studies were conducted to evaluate the light stability of the formulation (absorption spectrum, emission spectrum, measurement of Fourier transform infrared spectroscopy) and bioimaging.The experimental results show that: CdSe, DSPE-PEG-CdSe quantum dots have a higher degree of crystallinity, particle size of 3 ~ 5nm. PEG-modified quantum dot absorption peaks showed 0 nm shift, MPA and CYS modified quantum dot absorption peaks showed 3 nm and 5 nm red shifts, respectively.It was found that PEG chains and surface charges of quantum dots play a key role in cellular uptake and that MCF-7 breast cancer cells were used as a cellular uptake model and found that anionic QDs had higher uptake than cationic QDs formulations.In general, QDs with different surface ligands have some degree of strength in one aspect.Therefore, the type of QDs formulation should be carefully considered and chosen in the experiment, thus minimizing the impact of its limitations.
Keywords: Quantum dots Surface ligands Biological imaging Optical properties
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