宏基因组学方法研究β-内酰胺类有抗奶对犊牛瘤胃菌群结构的影响
首发时间:2018-10-17
摘要:(目的)本文旨在用宏基因组学方法研究β-内酰胺类有抗奶对犊牛瘤胃细菌菌群结构的影响。(方法)选用54头刚出生的犊牛随机分成3组,分别为对照组 (CK),饲喂无抗鲜奶组;β-内酰胺类有抗奶组(AM);巴氏消毒β-内酰胺类有抗奶组(APM)。试验期180 d。每组分别于60 d(T1),90 d(T2) 和180 d(T3)取犊牛瘤胃液用于犊牛瘤胃细菌宏基因组文库的构建。(结果):结果表明(1)9个样品的高质量 reads 占下机 reads 的百分比和高质量序列碱基占下机碱基的百分比均超过96%。(2)获得的reads碱基在10%-90%区间碱基Q值在35-40区间。(3)9个样品丰度最高的细菌为拟杆菌门(Bacteroidetes,平均丰度=69.8%),其次是厚壁菌门(Firmicutes,平均丰度=9.8%)。(结论)通过筛选,确定了纤维杆菌门和杆菌属这两种菌在犊牛瘤胃中对消化营养物质起重要的作用,拟杆菌对含有β-内酰胺类抗生素的巴氏灭菌奶更敏感。β-内酰胺类抗生素有抗奶可能通过影响螺旋体属细菌导致与食品安全相关的耐药性问题。
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Metagenomics study on the effect of β-lactam antibiotic on the structure of rumen bacteria in calves
Abstract: (Objective) This paper aims to study the effect of β-lactam antibiotic on the structure of rumen bacteria in calves by metagenomics. (Method) In this experiment, 54 newborn calf were selected and randomly divided into 3 treatments according to the principle of similar birthday age. The test was divided into: 1) control group (CK), feeding no antibiotic milk group; 2) antibiotic milk group (AM); 3) pasteurization antibiotic milk group (APM). The trial period was 180 days.The rumen bacteria genomic library of calf was constructed from 60 g (T1), 90 d (T2) and 180 d (T3) calf rumen fluids in each group. (Results): The results indicate that (1) The high-quality reads of the nine samples accounted for more than 96% of the percentage of reads and high-quality sequence bases. (2)The reads bases obtained were in the 10%-90% range and the Q value was in the 35-40 range. (3) The bacteria with the highest abundance of 9 samples were Bacteroidetes (average abundance=69.8%), followed by Firmicutes (average abundance=9.8%). (Conclusion) Through screening, it was determined that the two bacteria of the rumen of the calf rumen played an important role in the digestion of nutrients in the rumen. Bacteroides for pasteurized milk containing β-lactam antibiotics More sensitive. The antibiotic milk with β-lactam antibiotic may cause food-safety related drug resistance problems by affecting spirulina bacteria.
Keywords: calf rumen antibiotic milk flora metagenomics
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宏基因组学方法研究β-内酰胺类有抗奶对犊牛瘤胃菌群结构的影响
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