Study on analgesic effect of Anemoside B4

• 1、College of Pharmacetical Science,Soochow University,Suzhou 215123,china

Abstract：Objective: To investigate the role and influence of Anemoside B4 in pain and provide experimental evidence for its clinical application. Methdds: The behavioral changes of mice after administration in different dose groups were observed by hot plate, thermal radiation , Von Frey cilia mechanical stimulation, acetic acid writhing and formaldehyde experiment, investigated the analgesic effect of Anemoside B4. Results: The results show that in the thermal radiation, the Anemoside B4 of high dose group (6 mg/kg) could increase the threshold of pain in mice at 90 min.In the mechanical stimulation experiment, the high dose group could increase the pain threshold of mice at 60 min and 90 min.In the acetic acid writhing test, the number of writhing in the mice of each dose group of Anemoside B4 was significantly reduced compared with the model.There was a significant difference in the reduction of writhing times in the high-dose group (6 mg/kg), and the low-dose group (3 mg/kg) was statistically significant, the data was dose-dependent,and the number of writhings in the positive control group (Aspirin) was also significantly reduced, and the data had significant differences;In the writhing test combined with naloxone and atropine, the analgesic effect of Anemoside B4 could not be blocked by naloxone and atropine;In the formalin-induced inflammatory pain model, Anemoside B4 significantly reduced the time and number of licking in the second phase of the mouse.Conclusion: Anemoside B4 has good analgesic effect on thermal radiation pain, mechanical pain,acetic acid-induced mouse writhing model and formalin-induced mouse pain model.

Keywords： Anemoside B4 analgesia inflammatory pain