Molecularly imprinted polymers based on carbon dots for highly selective detection of chloramphenicol in crucian

• 1、School of food science and technology,Jiangnan University, Wuxi 214122

Abstract：Carbon dots (CDs) as fluorescent probes and molecularly imprinted membrane (MIP) as enrichment container for specific detection of chloramphenicol (CAP) was developed as a novel fluorescence sensor MIP@CDs. The prepared fluorescence sensor was synthesized by reversed microemulsion, which had an excellent dispersibility and stability in water. The fluorescence sensor also had a high sensitivity to the target substance (i.e. CAP), and the reacting equilibrium was able to be achieve within 5 min. The linear range of quantitative detection was ranging from 0.005 to 40 μg/mL with the limit of detection was 4.236 μg/L (3σ/Ksv). In addition, compared with non-imprinted polymers-coated CDs (NIP@CDs) and CDs, MIP@CDs had a better quantitative ability for detecting CAP at a relative low concentration. Furthermore, MIP@CDs showed a better selectivity for CAP compared with other antibiotics. Feasibility of this method on detecting CAP in crucian was successfully conducted; and the recoveries were calculated as 90.02-102.53%.

Keywords： carbon dots molecularly imprinted polymers fluorescence sensor chloramphenicol