Urinary proteomics strategies for urothelial bladder cancer: in mass spectrometry-based

• 1、 Institute of Urology, Lanzhou University Second Hospital, Key Laboratory of Gansu Province for Urological Diseases, Gansu Nephro-Urological Clinical Center, Lanzhou 730030, P.R. China
• 2、Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, Lanzhou 730000, P.R. China

Abstract：Urothelial bladder cancer is one of the most common urinary tract carcinomas. The earlier this cancer is found and treated, the better the outcome. UBC detection and surveillance include cytology, which suffers from low sensitivity, particularly for low-grade tumors, and cystoscopy, which is invasive and expensive. There is therefore a clinical need for biomarkers via a quick urine test to rapidly and accurately diagnose UBC Review of urinary proteomics strategies for urothelial bladder cancer: in mass spectrometry-basedand its relapse. Urine proteomic research is an effective way to find candidate biomarkers, which has been explored for many years. Based on the inherent complexity of proteomics and diverse methods of mass spectrometry analysis, existing research results from different groups are often confused and not easily referenced. Hence, with the development of mass spectrometry technology, this field still need to be explored constantly in new candidate finding and verification. In this review, three steps of mass spectrometry analysis, collection and preservation of urine specimens, total protein extraction and mass spectrometry data collection, involved in urine proteome analyzing of urothelial bladder cancer were mentioned and discussed to provide a theoretical reference for clinical and scientific researchers.?????

Keywords： Urothelial bladder cancer, urine proteome, tumor markers Mass spectrometry, sample preparation, data collection