IRF1对放射性皮肤损伤的防护作用及机制研究
首发时间:2019-01-11
摘要:目的:探讨干扰素调节因子-1(IRF1)对放射性皮肤损伤进展的影响。方法:采用免疫荧光和Western Blot检测人永生化表皮细胞(HaCaT)IRF1表达和分布的变化;采用MTT、EDU和细胞克隆实验检测细胞的增殖能力;采用流式细胞计数法检测细胞凋亡率;采用LDH法检测细胞毒性;采用IRF1基因敲除小鼠,建立小鼠放射性皮肤损伤模型,检测IRF1对放射性皮肤损伤进展的影响;采用RNA芯片分析IRF1基因影响的RNA。结果:受照后的皮肤HaCaT细胞中的IRF1表达升高;在HaCaT中过表达IRF1后,细胞的增殖能力减弱,克隆形成减少,细胞的凋亡率增加。动物水平上,IRF1基因纯合缺失小鼠在受到电离辐射后皮肤损伤较轻。结论:干扰素调节因子-1(IRF1)参与了放射性皮肤损伤的进展,并有望作为放射性皮肤损伤的防治新靶点。
关键词: 干扰素调节因子-1(IRF1) 电离辐射 放射性皮肤损伤。
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The radioprotective effect and mechanism of IRF1 on radiation-induced skin injury
Abstract:Objective: To investigate the effect of interferon regulatory factor-1 (IRF1) on the progression of radiation-induced skin lesions. Methods: The expression and distribution of IRF1 in human immortalized epithelial cells (HaCaT) were detected by immunofluorescence and Western Blot. The proliferation of cells was detected by MTT, EDU and cell cloning experiments. The apoptosis rate was detected by flow cytometry. The cytotoxicity was detected by LDH-based method. The model of radiation-induced skin damage was established by using IRF1 knockout mice to detect the effect of IRF1 on the progression of radiation-induced skin damage. The RNA affected by IRF1 gene was analyzed by RNA chip. RESULTS: The expression of IRF1 was up-regulated in HaCaT cells after irradiation. After overexpression of IRF1 in HaCaT cells, the proliferation of cells was reduced and the formation of clones was decreased, whereas the apoptosis rate of cells was increased. At the animal level, mice with homozygous deletion of the IRF1 gene had less severe skin damage after exposure to ionizing radiation. Conclusion: Interferon regulatory factor-1 (IRF1) is involved in the development of radiation-induced skin lesions and is expected to serve as a new target for the prevention and treatment of radiation-induced skin lesions.
Keywords: Interferon regulatory factor 1(IRF1) Ionizing radiation Radioactive skin injury
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