益生菌对镉暴露造成的小鼠肠道动力损伤缓解的机制
首发时间:2019-01-14
摘要:镉(Cadmium,Cd)是一种在环境中广泛存在的重金属,会对人体健康构成威胁。前期研究发现植物乳杆菌CCFM8610对镉有良好的吸附能力。本研究拟探究植物乳杆菌CCFM8610缓解镉暴露造成的小鼠肠道动力损伤的机制。将40只4周龄的C57BL/6J小鼠随机分为四组:空白组、模型组、干预组、CCFM8610灌胃组。小鼠通过饮水暴露于重金属镉环境10周,实验期间收集小鼠粪便,检测粪便含水量;实验结束后,取肝脏、肾脏、血液、结肠、空肠,测定镉含量、细胞因子、粪便菌群以及C-kit含量。结果表明,植物乳杆菌CCFM 8610 能有效减少肠道对重金属镉的吸收。同时植物乳杆菌CCFM8610 还调节镉暴露引起的肠道细胞自噬,肠道菌群紊乱,肠道内神经递质变化以及肠道Cajal细胞损伤。说明植物乳杆菌CCFM8610可以作为一种新型功能性食品添加剂。
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The damage of cadmium exposure on intestinal function and the alleviating mechanism of probiotics
Abstract:As a pollutant, Cadmium (Cd) is widely spread in the environment. It has been shown that Lactobacillus plantarum CCFM8610 can remove Cd from organism in our previous study. In which we found constipation in Cd-exposed mice while the mechanisms underlying the effects of L. plantarum CCFM8610 was unknown. Through drinking water, mice were exposed to Cd and orally administered 10-week L. plantarum CCFM8610. We also estimated the levels of Cd in blood, liver and kidney tissues and have made notes of alterations to several biomarkers of Cd toxicity. As a result, it was shown that when Cd was introduced orally, probiotics relieved Cd-induced intestinal motility disorder in mice gut, co-treatment with L. plantarum CCFM8610 effectively decreased intestinal Cd absorption. Moreover, L. plantarum CCFM8610 modulated Cd-induced autophagy, gut flora instability, changes in neurotransmitters and damage to Cajal cells. L. plantarum CCFM8610 is suggested to be developed into a new supplement of functional food in the results.
Keywords: probiotics heavy metal gastrointestinal motility Cajal cells
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益生菌对镉暴露造成的小鼠肠道动力损伤缓解的机制
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