The effects of reduction of intestinal flora by antibiotics to cerulein-induced chronic pancreatitis in mice

• 1、School of Food Science and Technology, Jiangnan University, WuXi, P.R. China 214122
• 2、School of Medicine, Jiangnan University, WuXi, P.R. China 214122

Abstract：Objective: To investigate the effects of reduction of intestinal flora by antibiotics (Abx) on cerulein-induced chronic pancreatitis(CP) and its possible mechanism in mice. Methods: 8 weeks male C57BL/6J mice were randomly divided into control group, SCFA control group model control group, antibiotic treatment group and SCFA treatment group. Antibiotics were administered intragastrically for 7 days before modeling and throughout the model, then CP model was established by intraperitoneal injection of cerulein for 4 weeks. The level of fibrosis was determined by masson trichrome stain kits, and the levels of inflammatory factors and growth factors were tested by real-time polymerase chain reaction(qPCR). Histone deacetylases(HDACs) and fibrosis-related proteins were detected by Western blotting. Results: Abx can worsen the cerulein-induced CP in mice, which is characterized by increased pancreatic atrophy, loss of acinar cells and increased fibrosis-related proteins, as well as the activation of Smad3 pathway, reduction of intestinal short chain fatty acids(SCFAs), and up-regulated the expression of HDAC1 in pancreas. Conclusion: Reduction of intestinal flora by Abx can aggravate CP in mice and its mechanism may be related to the decrease of SCFAs and the expression of HDACs.

Keywords： chronic pancreatitis fibrosis short chain fatty acids histone deacetylase