载钇二氧化钛涂层的生物活性及其抗菌性能研究
首发时间:2019-03-12
摘要:植入部位的炎性反应是临床上常见的植入术后并发症之一,主要是细菌感染所致。制备具有抗菌性能的涂层是防止细菌感染的有效方法,且涂层需要有良好的生物相容性,从而确保植入物材料与骨良好的结合。本研究通过微弧氧化法在Ti6Al4V表面制备含钇的TiO2涂层。评价了其生物活性及抗菌性能,并与未掺钇组进行对比。使用SEM,XRD,XPS等对涂层的表面形态和结构进行表征。结果发现:掺钇并未改变涂层的形貌,亲水性和物相组成。细胞实验证明涂层具有良好的生物相容性。含钇的微弧氧化涂层可以促进MC3T3-E1和HGF的增殖。ALP和TGF-β的表达量均增加,茜素红染色钙结节的量也随着钇浓度掺入量的增加而增加。细菌实验证实了含钇涂层对金黄色葡萄球菌及大肠杆菌有一定的抗菌能力。这项研究首次揭示了载钇二氧化钛涂层的生物相容性及抗菌能力,从而研发了一种理想的新型骨科及牙科植入体修复材料,为骨组织修复与重建中材料的选取提供一种优选策略。
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Bioactive and antibacterial Y-doped TiO2 coating by plasma electrolytic oxidation on Ti6Al4V alloy
Abstract:Inflammatory response at implantation sites is one of the most common postoperative complications in clinical practices, mainly due to bacterial infections. Antibacterial coatings are effective approaches to preventing bacterial infections. Such coatings need to have a good biocompatibility to ensure that the implant materials are highly compatible with bones. In this study, Yttrium (Y)-doped TiO2 was coated on the surface of Ti-6Al-4V via plasma electrolytic oxidation (PEO), and its bioactivity and antibacterial properties were assessed and compared to the undoped group. The surface morphology and structure of coatings were characterized via scanning electron microscope (SEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), etc. Our results revealed that Y did not alter the morphology, roughness and phase composition of those coatings. The cell culture assay demonstrated that those coatings have a good biocompatibility. Both osteoprecursor cells and fibroblasts displayed greater proliferative capacities on Y-doped PEO coating. The expression levels of alkaline phosphatase (ALP) and transforming growth factor beta (TGF- β ) in osteoprecursor cells grown on the surface of those materials increased from day 1 to day 7. Besides, the number of alizarin red-stained calcified nodules also rose alongside increasing doping concentration of Y. The bacterial assay confirmed that those Y-doped coatings exhibit a certain degree of antibacterial capacity against Staphylococcus aureus and Escherichia coli. In this study, we revealed for the first time the biocompatibility and antibacterial capacity of Y-doped titanium dioxide (TiO2) coating and has developed a new ideal implant and reconstruction material in dentistry and orthopedics. Hence, our study has provided a preferred strategy for the selection of materials for the repair and reconstruction of bone tissues.
Keywords: oral implantology,bio-compatibility, antibiosis, yttrium, microarc oxidation
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