辛烯基琥珀酸淀粉酯对高脂饮食小鼠肠道内环境的影响
首发时间:2019-03-14
摘要:目的:研究红薯辛烯基琥珀酸(OSA)淀粉酯对高脂饮食小鼠体重的控制作用以及对肠道内环境的影响。方法:以C57BL/6小鼠为动物模型,分别给予普通饮食(RF),高脂饮食(HF)和添加红薯OSA淀粉的高脂饮食(HFOSA)。干预22周后,观察各组小鼠体重,采用气相色谱法测定粪便中的短链脂肪酸的含量,高通量测序分析粪便菌群组成。结果:与高脂对照组相比,OSA淀粉干预组小鼠体重显著下降,粪便中丙酸和丁酸占总短链脂肪酸的比例显著增加,Parabacteroides,Alistipes,Ruminiclostridium_5菌群丰度显著增加,Tyzzerella,Oscillibacter,Desulfovibrio, Lachnospiraceae_UCG-006等菌群丰度显著降低。结论:红薯OSA淀粉能够有效控制高脂饮食引起的小鼠体重增加,显著提高肠道发酵产物丙酸、丁酸的含量,并改变肠道菌群结构。
关键词: 食品营养学 短链脂肪酸 肠道菌群 16S rDNA 辛烯基琥珀酸淀粉酯 高脂饮食
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Effects of OSA modified starch on the intestinal environment of high-fat diet induced mice
Abstract:Effects of octenylsuccinic anhydride (OSA) modified starch on body weight and intestinal environment of C57BL/6 mice were carried out. Thirty mice were grouped and treated with regular fat (RF) diet, high fat (HF) diet and high fat diet supplemented with OSA starch (HFOSA). After 22 weeks of treatment, body weight was measured, fecal short chain fatty acids (SCFAs) were quantified using gas chromatography (GC) and fecal microbiota was analyzed by 16S rDNA. Compared to HF group, body weight was significantly lower in HFOSA group. Propionic acid and butyric acid content in feces of mice in HFOSA group were significantly increased. The relative abundance of Parabacteroides,Alistipes,Ruminiclostridium_5 were increased and Tyzzerella,Oscillibacter,Desulfovibrio, Lachnospiraceae_UCG-006 were decreased. In conclusion, OSA starch had a positive effect on controlling body weight of mice under high fat diet. Furthermore, OSA starch could help to significantly increase the amount of propionic acid and butyric acid and change fecal microbiota composition.
Keywords: Food nutrition short chain fatty acids intestinal microbiota 16S rDNA OSA starch high fat diet
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