氯喹诱导博来霉素及奈达铂对舌鳞癌SCC25细胞凋亡的影响

王若宇; 杨兰

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氯喹诱导博来霉素及奈达铂对舌鳞癌SCC25细胞凋亡的影响

首发时间：2019-03-15

王若宇 ^{1
2}
王若宇，男，硕士在读，主要从事口腔颌面外科临床及科研工作
杨兰 ^{1
2}
杨兰，博士，教授

1、兰州大学口腔医学院
2、兰州大学第二医院口腔颌面外科兰州 730030，甘肃

摘要：目的: 研究氯喹诱导化疗药博来霉素和奈达铂对舌鳞癌SCC25细胞胞凋亡的影响及机制。方法：应用MTT法筛选不同药物对SCC25细胞生长的抑制作用，采用MTT、Hoechst33258染色、FCM观察不同药物不同处理方式后对SCC25细胞形态、细胞周期以及凋亡的变化，Western blot定量分析氯喹诱导不同化疗药物对SCC25细胞中Bax、Bcl-2、NFkB凋亡相关蛋白表达的影响。结果：经氯喹诱导后的博来霉素及奈达铂组SCC25细胞的增殖抑制率明显高于单独博来霉素及奈达铂组（p＜0.05）；Hoechst33358荧光染色和流式结果表明与博来霉素组比，氯喹加博来霉素组凋亡率显著提高，与奈达铂单独作用相比，氯喹+奈达铂组细胞凋亡率显著提高（p＜0.05）。氯喹+博来霉素组细胞周期G0/G1期所占比例高于博来霉素组（p＜0.05），S期与G2/M期细胞比例降低；同样氯喹+奈达铂组G0/G1期所占细胞比例高于奈达铂组（p＜0.05），S期与G2/M期细胞比例降低。 WB结果显示氯喹+博来霉素组Bax、NFkB表达水平显著高于单纯博来霉素组，Bcl-2表达水平则低于单纯博来霉素组；而氯喹+奈达铂组Bax、NFkB表达水平明显高于单纯奈达铂组，Bcl-2表达水平低于单纯奈达铂组，差异具有显著性（p＜0.05）。结论：氯喹诱导博来霉素及奈达铂对舌鳞癌SCC25细胞的增殖具有显著的抑制作用，其促凋亡作用可能与线粒体凋亡相关蛋白和核转录因子的活化相关。

关键词：氯喹博来霉素奈达铂舌鳞癌SCC25细胞增殖凋亡周期

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Effect of chloroquine on the apoptosis of tongue squamous cell carcinoma SCC25 cells induced by bleomycin and nedaplatin

wangruoyu ^{1
2}
王若宇，男，硕士在读，主要从事口腔颌面外科临床及科研工作
yanglan ^{1
2}
杨兰，博士，教授

1、1 School of Stomatology, Lanzhou University
2、 2 Department of Oral and Maxillofacial Surgery, Second Hospital of Lanzhou University, Lanzhou 730030, China

Abstract：Objective: To study the effect and mechanism of chloroquine-induced chemotherapy drugs bleomycin and nedaplatin on apoptosis of tongue squamous cell carcinoma SCC25 cells.METHODS: The inhibitory effect of different drugs on the growth of SCC25 cells was screened by MTT assay. MTT, Hoechst33258 staining and FCM were used to observe the changes of morphology, cell cycle and apoptosis of SCC25 cells after different treatments. The quantitative analysis of chloroquine induced by Western blot The effect of chemotherapy drugs on the expression of Bax, Bcl-2 and NFkB apoptosis-related proteins in SCC25 cells. RESULTS: The proliferation inhibition rate of bleomycin and nedaplatin SCC25 cells induced by chloroquine was significantly higher than that of bleomycin and nedaplatin alone (p<0.05); Hoechst33358 fluorescence staining and flow cytometry showed In the bleomycin group, the apoptotic rate of the chloroquine plus bleomycin group was significantly increased, and the apoptosis rate of the chloroquine + nedaplatin group was significantly higher than that of the nedaplatin group (p<0.05). The proportion of G0/G1 phase in the chloroquine + bleomycin group was higher than that in the bleomycin group (p<0.05), and the ratio of cells in the S phase to the G2/M phase was decreased; the same chloroquine + nedaplatin group G0/G1 The proportion of cells in the period was higher than that in the nedaplatin group (p<0.05), and the proportion of cells in the S phase and G2/M phase decreased. WB results showed that the expression levels of Bax and NFkB in chloroquine + bleomycin group were significantly higher than those in bleomycin group alone, and the expression level of Bcl-2 was lower than that in bleomycin group alone. Bax and NFkB in chloroquine + nedaplatin group The expression level was significantly higher than that of the nedaplatin group alone. The expression level of Bcl-2 was lower than that of the nedaplatin group alone, and the difference was significant (p<0.05). Conclusion: Chloroquine induced bleomycin and nedaplatin can significantly inhibit the proliferation of tongue squamous cell carcinoma SCC25 cells, and its proapoptotic effect may be related to the activation of mitochondrial apoptosis-related proteins and nuclear transcription factors.

Keywords： chloroquine bleomycin nedaplatin tongue squamous cell carcinoma SCC25 cells proliferation apoptosis cycle

基金：

1. 兰州大学第二医院博士生/硕士生科研基金资助项目（sdkygg-17）

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王若宇，杨兰. 氯喹诱导博来霉素及奈达铂对舌鳞癌SCC25细胞凋亡的影响[EB/OL]. 北京：中国科技论文在线 [2019-03-15]. https://www.paper.edu.cn/releasepaper/content/201903-190.

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论文编号	201903-190
论文题目	氯喹诱导博来霉素及奈达铂对舌鳞癌SCC25细胞凋亡的影响
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