Antibacterial Activity research of Nitrogen Doped Graphene Nanomaterials

• 1、Soochow University，suzhou，215123

Abstract：Bacterial resistance has become a global problem, and it is imperative to solve this problem. To this end, we synthesized a carbon-nitrogen-doped two-dimensional nanomaterial N-Gra. First, N-Gra were characterized by TEM, FITC and XPS. The results show that the average size of N-Gra is about 5 nm. Next, a bacterial colony integration experiment (CFU) was used to determine the toxicity of N-Gra to bacteria. SEM and TEM were used to observe changes in bacterial morphology after N-Gra treatment. The results show that N-Gra has a broad-spectrum antibacterial effect, but its damage to bacterial membrane is not obvious. The activity status of bacteria after N-Gra treatment was verified by Live-dead, and the change of bacterial value-adding ability was observed. It was found that N-Gra may affect the proliferation ability of bacteria. In addition, we also evaluated the biotoxicity of N-Gra on umbilical vein endothelial cells (HUVEC) and human lung epithelial cells (Bease-2B), and found that N-Gra is mildly toxic to eukaryotes. These findings may provide a basis for better design of new nanomaterial antibacterial agents.

Keywords： N-Gra antibacterial mechanism proliferative capacity cytotoxicity