少根紫萍和多根紫萍淀粉结构及理化性质的研究
首发时间:2019-03-21
摘要:本研究以寡营养培育的少根紫萍和多根紫萍为材料,经提取获得了少根紫萍和多根紫萍淀粉,以玉米淀粉和马铃薯淀粉作对照,通过扫描电子显微镜、X-射线衍射仪、高效阴离子交换色谱、快速黏度分析仪等现代分析仪器对淀粉进行结构和理化性质的比较研究。结果表明:两种浮萍淀粉在颗粒大小,结晶结构及热力学性质上存在显著差异,少根紫萍淀粉分为大、小两种颗粒,B型结晶,而多根紫萍淀粉颗粒大小均一,A型结晶,初始糊化温度高于少根紫萍淀粉;与对照淀粉共同对比发现:玉米淀粉和马铃薯淀粉在颗粒大小、晶型、糊化特性等方面差异显著;与马铃薯淀粉相比,多根紫萍淀粉与玉米淀粉晶型同为A型,短程有序性低,支链链长分布上短链段多,糊化性质相似;与玉米淀粉相比,少根紫萍淀粉与马铃薯淀粉同为B型结晶,支链链长分布上长链段多,但糊化性质上少根紫萍淀粉与玉米淀粉相似,其峰值黏度、崩解值、回生值均低于马铃薯淀粉。
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Study on structure and physicochemical properties of the starches from Landoltia punctata and Spirodela polyrhiza
Abstract:By taking oligotrophic Landoltia punctata and Spirodela polyrhiza as materials in this research, Landoltia punctata and Spirodela polyrhiza starches are acquired after extraction. Comparative study is conducted on structure and physiochemical properties of starches with modern analytical instruments such as scanning electron microscope, X-ray diffraction,high performance anion exchange chromatography andrapid viscosity analyzerby taking corn starch and potato starch as contrast. According to research findings, there are significant differences on granular sizes, crystalline structures, and thermodynamic properties between the two types of duckweed starches. Landoltia punctata starch includes two types of particles, i.e., big size and small size, with B-type crystals; however, Spirodela polyrhiza starch has uniform size, with A-type crystals and higher initial gelatinization temperature than that of Landoltia punctata starch. Based on comparisons made to the control starches, there are significant differences between corn starch and potato starch on granular sizes, crystal forms, and gelatinization properties. Compared with potato starch, both Spirodela polyrhiza starch and corn starch have A-type crystal form, with low short range ordering, multiple short chain sections distributed in length of branched chains, and similar gelatinization properties. Compared with corn starch, both Spirodela polyrhiza starch and potato starch have B-type crystal form, with multiple long chain sections distributed in length of branched chains. However, the Landoltia punctata starch has similar gelatinization properties with corn starch, and its peak viscosity, breakdown value, and setback value are all lower than that of potato starch.
Keywords: Landoltia punctata Spirodela polyrhiza starch structure physicochemical properties
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