益生菌缓解流感病毒引起呼吸道感染的作用研究
首发时间:2019-03-22
摘要:流感病毒具有高度变异性,目前还缺乏长期有效的防治方法。本研究中,通过建立流感病毒导致的呼吸道感染动物模型,比较分析不同种益生菌对流感型呼吸道感染的缓解作用。结果显示:H1N1流感病毒感染后,模型组小鼠体重显著下降,青春双歧杆菌和短双歧杆菌可有效缓解流感引起的体重降低;青春双歧杆菌、短双歧杆菌、鼠李糖乳杆菌、植物乳杆菌、发酵乳杆菌不同程度的改善小鼠肺部病理指标,短双歧干预组的炎症浸润较少且支气管相对完整,与药物治疗相似。同时,短双歧杆菌可显著增加抗病毒蛋白MxA的表达量,降低小鼠肺部病毒载量;而植物乳杆菌组和发酵乳杆菌可显著增加抗病毒蛋白Oas1a的表达量。肠道菌群的16S RNA宏基因组分析发现,不同种益生菌对菌群结构及其代谢产物的影响存在菌种特异性。本研究发现了益生菌缓解流感病毒引起呼吸道感染的菌种差异性,同时建立了肠道菌群和抗流感病毒感染的潜在关联。
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Oral administration of probiotics alleviates the respiratory inflammation caused by influenza virus
Abstract:Influenza viruses are highly variable and there is no effective prevention method. In this study, an animal model of respiratory tract infection caused by influenza virus was established to compare and analyze the mitigation effects of different probiotics on influenza-type respiratory infection. The results showed that after H1N1 influenza virus infection, the body weightwas significantly decreased in model group. Bifidobacterium adolescentis group and Bifidobacterium breve group could effectively alleviate the weight loss caused by influenza; pathological sections of the lungs showed Bifidobacterium adolescentis, Bifidobacterium breve, Lactobacillus rhamnosus, Lactobacillus plantarum, and Lactobacillus fermentum improved lung lesions in different degrees. Bifidobacterium breve group had less inflammatory infiltration and relatively complete bronchus, similar to drug treatment group. At the same time, Bifidobacterium breve group can significantly increase the expression of antiviral protein MxA and reduce the viral load in the lungs of mice; while Lactobacillus plantarum group and Lactobacillus fermentum group can significantly increase the expression of antiviral protein Oas1a. The 16S RNA metagenomic analysis of the intestinal flora revealed that the effects of different probiotics on the structure of the flora and its metabolites were species-specific. These results indicate that different probiotics have different pathways to intervention on influenza infection. This study identified the potential relationship between microbiome and anti-influenza infection.
Keywords: probiotics respiratory infection influenza virus gut microbiome
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