云南泡梨中乳酸菌种群分析及其在泡梨发酵中的应用
首发时间:2019-03-29
摘要:为了对云南传统食品泡梨中乳酸菌进行系统分析并研究其在泡梨发酵中的功能,本文从不同来源传统泡梨中采用通用乳酸菌分离,鉴定的方法结合16S rDNA序列分析进行泡梨乳酸菌种群分析。选取产酸能力强的乳酸菌进行复合乳酸菌接种发酵的方法进行泡梨的制备并对制备的泡梨进行感官评价及其中风味成分的分析。结果表明在传统泡梨中乳酸菌含量丰富,其中植物乳杆菌为优势菌。复合乳酸菌发酵制备的泡梨其风味物质较自然发酵泡梨中更为丰富,使得复合乳酸菌发酵泡梨具有更加优秀的风味。这些结果表明乳酸菌是泡梨发酵的重要微生物,对泡梨中乳酸菌的深入研究与分析对泡梨的发酵机制,制备工艺和进一步标准化生产均具有理论意义和现实意义。
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Lactic Acid Bacteria in Pickled Pear and its Application
Abstract:In this paper, to analyze the lactic acid bacterial(LAB) strains in the pickled pear and study their function in the pickled pear fermentation, LAB were screened from tranditional pickled pear samples collected from different areas of Yunnan province. These strains were identified by morphological, physiological and biochemical and 16S rDNA sequencing. Strains with producing more lactic acid were used in pear fermentation, and the resulting products were evalued. And the volatile components in pickled pear were also analyzed. The results showed that there are abundant LAB in pickled pear, with Lactobacillus plantarum being the dominant member. The isolated LAB were used as starter in fermenting pear production, and their effects on the pear flavors were analyzed, which showed much more volatile components in pickled pear fermented with LAB inoculation than that in the natural fermented pear. The volatil components confered the pear better flavor. These results showed that LAB is important in pickled pear production, and study of these bacteria and their function in the pear fermentation is important for the fermentation mechanism study, process improvement and standardized production of pickled pear.
Keywords: fermentation pickled pear lactic acid bacteria physiological and biochemical characteristics isolation and identification.
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云南泡梨中乳酸菌种群分析及其在泡梨发酵中的应用
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