PER1对人肝癌细胞BEL-7402辐射敏感性的影响

潘书贤; 肖昌浩; 黄皓; 胡文涛

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PER1对人肝癌细胞BEL-7402辐射敏感性的影响

首发时间：2019-04-29

潘书贤 ¹
潘书贤，男，硕士，硕导：周光明，肿瘤免疫
肖昌浩 ¹
肖昌浩，男，硕士，硕导：柴之芳，肿瘤放疗
黄皓 ¹
黄皓，男，硕士，硕导：周光明，肿瘤放疗
胡文涛 ¹
胡文涛，男，讲师，放射生物

1、苏州大学放射与防护学院，苏州，215123

摘要：目的：研究Per1在人肝癌细胞辐射敏感性中的作用。方法：X射线照射SMMC-7721、BEL-7402和HepG2肝癌细胞后，克隆形成实验检测其存活分数；Western blot和 RT-qPCR 检测Per1在肝癌细胞系中的表达；X射线照射过表达Per1的肝癌细胞，克隆形成实验检测存活分数；流式细胞仪检测凋亡。结果：在梯度剂量X射线0、2、4、6、8、10Gy照射下SMMC-7721，BEL-7402和HepG2克隆存活分数逐渐下降，其中SMMC-7721对辐射最敏感，BEL-7402辐射抗性最高。Western blot检测发现Per1在SMMC-7721中的表达水平明显高于BEL-7402和HepG2（p<0.05）。过表达Per1蛋白以后，BEL-7402接受5Gy X射线照射后凋亡明显增多，同时，Western blot 和RT-PCR实验结果发现，X射线照射过表达Per1的BEL-7402细胞，抗凋亡蛋白Bcl-2表达明显降低，促凋亡蛋白Caspase3表达明显升高。结论：PER1蛋白的高水平表达可以促进X射线诱导的凋亡，增强肝癌细胞的辐射敏感性。

关键词：肝癌昼夜节律调节蛋白辐射敏感性放疗

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Effect of PER1 on Radiation Sensitivity of Human Hepatoma Cell Line BEL-7402

Pan shuxian ¹
潘书贤，男，硕士，硕导：周光明，肿瘤免疫
Xiao changhao ¹
肖昌浩，男，硕士，硕导：柴之芳，肿瘤放疗
Huang hao ¹
黄皓，男，硕士，硕导：周光明，肿瘤放疗
Hu wentao ¹
胡文涛，男，讲师，放射生物

1、School of Radiation and Protection, Suzhou University, Suzhou, 215123

Abstract：objective: To study the role of Per1 in the radiation sensitivity of human hepatocellular carcinoma. Methods: MMC-7721, BEL-7402 and HepG2 hepatoma cells were irradiated by X-rays, and the survival fraction was detected by colony formation assay. Western blot and RT-qPCR were used to detect the expression of Per1 in hepatocellular carcinoma cell lines and the expression of apoptosis-related proteins. Hepatoma cells overexpressing Per1 were irradiated by X-ray, and the survival fraction was detected by colony formation assay. Apoptosis was detected by flow cytometry. Results: The survival fraction of SMMC-7721, BEL-7402 and HepG2 clones decreased gradually under gradient dose X-rays of 0, 2, 4, 6, 8, 10 Gy. SMMC-7721 was the most sensitive to radiation and BEL-7402 radiation resistance highest. Western blot analysis showed that the expression level of Per1 in SMMC-7721 was significantly higher than that of BEL-7402 and HepG2 (p<0.05). After overexpression of Per1 protein, apoptosis of BEL-7402 was significantly increased after 5Gy X-ray irradiation. At the same time, Western blot showed that the expression of anti-apoptotic protein Bcl-2 was significantly decreased and the expression of pro-apoptotic protein Caspase3 was significantly increased by X-ray irradiation of Per1 overexpressing BEL-7402 cells. Conclusion: High expression of PER1 protein can promote apoptosis induced by X-ray and enhance the radiation sensitivity of liver cancer cells.

Keywords： Hepatocellular carcinoma；Period circadian regulator 1；Radiosensitivity；Radiotherapykey word 3

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潘书贤，肖昌浩，黄皓，等. PER1对人肝癌细胞BEL-7402辐射敏感性的影响[EB/OL]. 北京：中国科技论文在线 [2019-04-29]. https://www.paper.edu.cn/releasepaper/content/201904-346.

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论文编号	201904-346
论文题目	PER1对人肝癌细胞BEL-7402辐射敏感性的影响
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