The effect of abnormal hematopoietic system induced by autophagy on the digestive system

• 1、Hematology Center of Cyrus Tang Medical Institute,Soochow University,Suzhou 215123
• 2、Medical college,Soochow University,Suzhou 215123

Abstract：Objective：To investigate the effects of abnormalities of the hematopoietic system caused by autophagy on the digestive system. Methods: 1) PCR and gel electrophoresis were used to identify Atg7f/f; Vav-iCre mouse genotype. 2) Mouse intestinal tissue was obtained and its length was measured. 3) HE staining technique was used to detect the histomorphological structure of stomach and intestine of mice. 4) RNA was extracted from the stomach and intestine of mice, and the activity of telomerase was detected by real-time fluorescence quantitative (Q-PCR) technique. 5) The DNA damage and P15 protein expression in the stomach and intestine tissues of mice were detected by Western blotting. Results: 1) Atg7f/f; Vav-iCre model mice were successfully constructed. 2) After the loss of Atg7 gene in hematopoietic system, the length of intestinal tissue of KO mice did not change significantly. 3) After the loss of Atg7 gene in hematopoietic system, HE staining showed that the number of small intestine villi was not reduced and the structure was not significant changes, no obvious lesions in the stomach tissue structure. 4) After the loss of the Atg7 gene in the hematopoietic system, the telomerase-related gene transcription levels in the stomach and intestine tissues of the mice did not change significantly. 5) After Atg7 gene deletion in hematopoietic system, there was no significant change in the phosphorylation of the DNA damage marker γ-H2AX protein and the expression of P15 protein in mouse stomach and intestinal cells. Conclusion: Hematopoietic abnormalities caused by autophagy have no significant effect on the digestive system.

Keywords： Hematopoietic system Autophagy Digestive system