大豆根瘤响应低磷胁迫的转录组分析
首发时间:2019-04-04
摘要:根瘤是生长在大豆根部的特殊器官,它是由土壤中的根瘤菌与豆科植物根相互作用形成的共生体,能够进行生物固氮。生物固氮是自然界最有效最经济的提供氮源的方式。磷是影响大豆质量和产量的重要因素,同时也是根瘤生长发育的必需元素。前期的研究表明低磷胁迫能够影响根瘤的生长发育,质量和数量,进而影响生物固氮,但是分子机理尚不清楚。本研究利用高通量测序技术,在低磷和正常磷处理下,对磷低效大豆品种"牛毛黄"接种根瘤菌28天后的根瘤样品进行转录组测序,筛选出1440个在根瘤中受低磷响应的差异基因,其中885个表达上调基因,555个表达下调基因。通过分析这些差异基因进而解析低磷胁迫下影响根瘤生长发育和生物固氮的信号通路和分子机理。
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mRNA-seq analysis of soybean nodules response to low phosphorus stress
Abstract:Root nodules are special organs growing on the soybean roots. They are symbionts formed by interaction between rhizobium bacteria in soil and root hairs of leguminous plants, which can perform biological nitrogen fixation. Biological nitrogen fixation is the most effective and economical way to provide nitrogen source in nature. Phosphorus is an important factor affecting the quality and yield of soybean. Previous studies have shown that low phosphorus stress can affect the growth, quality and quantity of root nodules, thus affecting biological nitrogen fixation, but the molecular mechanism is not clear. In this study, under low and normal phosphorus conditoins, 28-day root nodules of phosphorus inefficient soybean varieties "niumanghuang" were used for transcriptome sequencing by high-throughput sequencing technology and 1440 differential expression genes were identified with 885 up regulated and 555 down regulated. By analyzing the differential expression genes, it is expected to know the signaling pathways and molecular mechanisms between low phosphorus stress and biological nitrogen fixation.
Keywords: Low phosphorus stress nodule transcriptome differential expression genes
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大豆根瘤响应低磷胁迫的转录组分析
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