新型双光子激发/近红外发光的AIE探针用于细胞内Zn2+荧光成像
首发时间:2019-05-16
摘要:在本文中,我们合成了一种新型双光子激发/近红外发光的AIE(聚集诱导发光)荧光探针TPE-QB,用于水溶液和细胞内的锌离子检测。其作用原理是在新型近红外AIE荧光团上修饰的席夫碱基团能够与锌离子特异性络合,致使原本在水溶液中分散存在的TPE-QB聚集,从而发出很强的近红外荧光。TPE-QB的发射波长达到650 nm,对锌离子具有很好的选择性和很高的灵敏度。此外。它还具有很好的生物相容性,可用于细胞内锌离子单光子/双光子的检测成像和亚细胞水平的锌离子示踪。
关键词: 化学生物传感 聚集诱导发光 锌离子检测 近红外探针 双光子成像
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A Novel Two-Photon Excitation/Red-Emission AIE Chemsensor for Zn2+ Imaging Assay in Living Cells
Abstract:In this paper,a novel two-proton excited fluorescent sensor for the detection of zinc ion in aqueous solution as well as in cells was synthesized on the basis of complexation process using an aggregation-induced emission fluorophore. The near-infrared chemosensor possessing a schiff base derivatives with salicylaldehyde moiety show a distinctly selective response to zinc ion. The probe (TPE-QB) based on tetraphenylethene structure complexed zinc ion and then aggregated in aqueous buffered solution, causing remarkable enhancement (OFF-On) of red emissions at 650 nm. Furthermore, this fluorescent molecule easily penetrated cytomembrane and successfully achieved intracellular zinc ion imaging in living cells
Keywords: Biochemical Sensor Aggregation induced emission Zn2+ Monitor Near-Infrared Emissive Chemsensor Two-photon Imaging
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新型双光子激发/近红外发光的AIE探针用于细胞内Zn2+荧光成像
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